LOCUS       sequence-122991-        8352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for homologous recombination to insert a 2A-mCherry-NLS
            cassette in place of the stop codon of myogenin in the mouse 
            genome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8352)
  AUTHORS   Gersbach
  TITLE     TMG reporters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Myog'-2A-mCherryNLS-PGK-Purodtk
FEATURES             Location/Qualifiers
     source          1..8352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             964..1020
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1021..1728
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1166..1184)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1434..1453
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1729..1749
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1756..1776
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    1821..1854
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1915..2413
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1950..1973)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2346..2365
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      2427..2436
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2433..3032
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     2932..2952
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3039..4034
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     protein_bind    4035..4068
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(4785..4804)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4786..4804)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4811..4828)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4811..4827)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4820..4842)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4968..5396
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5055..5074)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5265..5286
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             5740..6534
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5794..5813)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6404..6423
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(6552..6620,6621..7412)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6770..6789)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7583..8171
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8072..8091
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8325..8342
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccgtg cccagtgaat gcaactccca cagcgcctcc tgcagtccgg
      301 agtggggcaa tgcactggag ttcggtccca acccaggagg taagtgaatc ctgaccctgg
      361 taacatggct caaatccctt agtccctacc taggctgggc ttgtaccctt accataagcc
      421 ctgtcccaat cacccttcag cagcttttct aggaacttgc tgacctgagg gccccaaatg
      481 gtgggctgga gaccctgtgc ctcccggtag ccttgaccag ctaaagagta gtcagagcct
      541 ccagcaactt ccctgctggt ctctgacctc agagagaaga gcctgctcct gccccagggg
      601 tccctgggga tcactcagtc agtgttgtaa aactgtgccc tgtgccctag gatatcctgg
      661 cttcacctag ggatagccac ttttaaaacc ccacaagcac acacacagca tggggagtac
      721 tagtgtcctg ggggcgggga acactcctga gtcttcctta cccattttgt cctggaaaat
      781 ctgtgtcaag ggtcccatac cagttgccca ggaaacattc tcccactttt tcttgcagat
      841 catttgctcg cggctgaccc tacagacgcc cacaatctgc actcccttac gtccatcgtc
      901 gacagcatca cggtggagga tatgtctgtt gccttcccag acgaaaccat gcccaacgct
      961 agcgccacta acttctccct gttgaaacaa gcaggggatg tcgaagagaa tcccgggcca
     1021 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
     1081 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     1141 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
     1201 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
     1261 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
     1321 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
     1381 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     1441 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     1501 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
     1561 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
     1621 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
     1681 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagcc caagaagaag
     1741 aggaaggtgg gtggccctaa gaaaaagaga aaggtgtgac gtacaagtaa gaattccgat
     1801 catattcaat aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc
     1861 tgaagaggag tttacgtcca gccaagctta ggatctcgac ctcgaaattc taccgggtag
     1921 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctggcacttg
     1981 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc
     2041 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
     2101 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
     2161 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
     2221 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
     2281 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
     2341 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
     2401 tccgggcctt tcgacctgca gccaacgcca ccatggggac cgagtacaag cccacggtgc
     2461 gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg
     2521 actaccccgc cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc
     2581 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     2641 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     2701 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     2761 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     2821 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     2881 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     2941 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     3001 gcacctggtg catgacccgc aagcccggtg ccggatccat gcccacgcta ctgcgggttt
     3061 atatagacgg tcctcacggg atggggaaaa ccaccaccac gcaactgctg gtggccctgg
     3121 gttcgcgcga cgatatcgtc tacgtacccg agccgatgac ttactggcag gtgctggggg
     3181 cttccgagac aatcgcgaac atctacacca cacaacaccg cctcgaccag ggtgagatat
     3241 cggccgggga cgcggcggtg gtaatgacaa gcgcccagat aacaatgggc atgccttatg
     3301 ccgtgaccga cgccgttctg gctcctcata tcggggggga ggctgggagc tcacatgccc
     3361 cgcccccggc cctcaccctc atcttcgacc gccatcccat cgccgccctc ctgtgctacc
     3421 cggccgcgcg ataccttatg ggcagcatga ccccccaggc cgtgctggcg ttcgtggccc
     3481 tcatcccgcc gaccttgccc ggcacaaaca tcgtgttggg ggcccttccg gaggacagac
     3541 acatcgaccg cctggccaaa cgccagcgcc ccggcgagcg gcttgacctg gctatgctgg
     3601 ccgcgattcg ccgcgtttac gggctgcttg ccaatacggt gcggtatctg cagggcggcg
     3661 ggtcgtggcg ggaggattgg ggacagcttt cggggacggc cgtgccgccc cagggtgccg
     3721 agccccagag caacgcgggc ccacgacccc atatcgggga cacgttattt accctgtttc
     3781 gggcccccga gttgctggcc cccaacggcg acctgtacaa cgtgtttgcc tgggccttgg
     3841 acgtcttggc caaacgcctc cgtcccatgc acgtctttat cctggattac gaccaatcgc
     3901 ccgccggctg ccgggacgcc ctgctgcaac ttacctccgg gatggtccag acccacgtca
     3961 ccacccccgg ctccataccg acgatctgcg acctggcgcg cacgtttgcc cgggagatgg
     4021 gggaggctaa ctgaataact tcgtataatg tatgctatac gaagttatgg cgcgccgatt
     4081 gtctgtcagg ctgggtgtgc atgtgagccc ccaagttggt gtcaaaagcc atcacttctg
     4141 tagcaggggg cttttaagtg gggctgtcct gatgtccaga aaacagccct gggctgccac
     4201 aagccagact ccccactccc cattcacata aggctaacac ccagcccagc gagggaattt
     4261 agctgactcc ttaaagcaga gagcatcctc ttctgaggag agaaagatgg agtccagaga
     4321 gcccccttgt taatgtccct cagtggggca aactcaggag cttctttttt gtttatcata
     4381 atatgcctcg aattccaccc cccaccccca aaatgaaacc gtttgagaga catgagtgcc
     4441 ctgacctgga caagtgtgca catctgttct agtctcttcc tgaagccagt ggctgggctg
     4501 ggcctgccct gagttgagag agaaggggga ggagctatcc ggttccaaag cctctggggg
     4561 ccaagcattt gcagtggatc ttgggaacct tccagtgctt tgtgtattgt ttattgtttt
     4621 gtgtgttgtt tgtaaagctg ccgtctgacc aaggtctcct gtgctgatga taccgggaac
     4681 aggcagggaa gggggtgggg gctcttgggg tgacttcttt tgttaactaa gcattgtgtg
     4741 gttttgcgcg gccgctcgag catgcatcta gagggcccaa ttcgccctat agtgagtcgt
     4801 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     4861 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     4921 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag
     4981 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     5041 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     5101 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     5161 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     5221 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     5281 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     5341 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     5401 caaaattcag ggcgcaaggg ctgctaaagg aagcggaaca cgtagaaagc cagtccgcag
     5461 aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca
     5521 agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg
     5581 gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg
     5641 aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga
     5701 tcaagatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     5761 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     5821 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     5881 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     5941 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     6001 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atcccacctt
     6061 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     6121 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     6181 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     6241 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     6301 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     6361 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     6421 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     6481 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaattgaa
     6541 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     6601 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6661 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6721 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6781 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6841 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6901 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6961 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7021 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7081 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7141 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     7201 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7261 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7321 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7381 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     7441 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     7501 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7561 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7621 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7681 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     7741 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7801 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7861 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7921 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7981 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8041 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8101 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8161 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8221 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8281 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8341 aggaagcgga ag
//
