LOCUS       sequence-123015-        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentivirus expressing mKO2 with UTR sensor cassette and hygromycin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: pGK:HygroR-CMV:mKO2-C_UTR
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(159..815)
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     promoter        complement(894..993)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(923..943)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gagcaacata gttaagaata ccagtcaatc tttcacaaat tttgtaatcc agaggttgat
       61 tatcgggcgc gccgcggtct agaagagtaa ttcaacccca aacaacaacg tttccatgca
      121 aggactataa atctcggcca tatgcctgag tggtatactc agctgtgggc cacggcatct
      181 tccacctgtt cggtgatgtt gccctcggtc ttgcgcacga gcctgtggcc gatgtagtgg
      241 tcgccgggca tttccaggat ttctttggcg gccttgtagg tggtcttcat ctggcacttg
      301 tggtttccgc cgccttccag cttcaggtac atggtcacgt cgcccttcag cacgccgtca
      361 gaggctgtga tcttctcggt gctgggctcc cagtccacag actggttctg catgatgggg
      421 ccgtcggcgg ggaagttcac gccggtgaac ttgctcttgt ggtagaaggt gttgcctctc
      481 agagagatgt gggcagacac agaggcgctg ccgccgtcct cgaattccag gcttctctcc
      541 cagctcaggc cctcggggaa ggcctgcttg aagtagtcgg ggatttcctc ggggtacttg
      601 gtgaacactc tgtggccgta gcagaacacg tgggacacca gatcgaaggc gaagggcata
      661 ggtccgccct cggccatggt cactctcagg gtcatttcct ggtggccctc gtaaggtctg
      721 cctgtgccct cgccctcgat ggtgaactcg tggccgttca cgctgccgtc catgtagtaa
      781 cgcatcttca tctcgggctt gatcacggac accatggtcc gcttaagctg tagcgctaat
      841 gtcgggtcaa tttcgataag ccagtaagca gtgggttctc tagttagcca gagagctctg
      901 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
      961 ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaac
//
