LOCUS       sequence-123044-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Liu F, Wagner S, Campbell RB, Nickerson JA, Schiffer CA, Ross AH
  TITLE     PTEN enters the nucleus by diffusion.
  JOURNAL   J Cell Biochem. 2005 Oct 1. 96(2):221-34.
  PUBMED    16088943
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biochem. 2005 Oct 1. 96(2):221-34."
COMMENT     Sequence Label: pcDNA3 GFP GFP
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(9..11,12..14,15..725)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(315..334)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     662..683
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cttagcttat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
       61 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      121 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      181 ggcccaccct cgtgaccacc ttcggctacg gcgtgcagtg cttcgcccgc taccccgacc
      241 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      301 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
      361 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
      421 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcacg gccgacaagc
      481 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
      541 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      601 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
      661 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
      721 acaagaagct tgcatgcctg caggtcgact ctagactgcc atggtgagca agggcgagga
      781 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
      841 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
      901 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
//
