LOCUS       sequence-123045-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rab7 with N-terminal FRB and triple myc tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Bentley M, Decker H, Luisi J, Banker G
  TITLE     A novel assay reveals preferential binding between Rabs, kinesins, 
            and specific endosomal subpopulations.
  JOURNAL   J Cell Biol. 2015 Feb 2;208(3):273-281. Epub 2015 Jan 26.
  PUBMED    25624392
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2015-02-2"; volume: "208(3)"; pages: "273-281. Epub 
            2015 Jan 26"
COMMENT     Sequence Label: pBa-FRB-3myc-Rab7
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             66..344
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
ORIGIN
        1 cttcccttct ctgttcctcc gcagccccca agcttggcgc gcctaggcct accatggctt
       61 ctagaatcct ctggcatgag atgtggcatg aaggcctgga agaggcatct cgtttgtact
      121 ttggggaaag gaacgtgaaa ggcatgtttg aggtgctgga gcccttgcat gctatgatgg
      181 aacggggccc ccagactctg aaggaaacat cctttaatca ggcctatggt cgagatttaa
      241 tggaggccca agagtggtgc aggaagtaca tgaaatcagg gaatgtcaag gacctcctcc
      301 aagcctggga cctctattat catgtgttcc gacgaatctc aaagactagt tatccgtacg
      361 gaatgcagaa gctgatctca gaggaggacc tgcaaaaact tatttctgaa gaggatctgc
      421 aaaagcttat ttctgaagaa gatctgtaca agcggaccgg tagtggcatg acctctagga
      481 agaaagtgtt gctgaaggtc atcatcctgg gggactctgg tgttggaaag acctctctca
      541 tgaaccagta tgtgaacaag aagttcagta accagtacaa agccacaata ggagcggact
      601 ttctgaccaa ggaggtgatg gtggacgaca gacttgttac catgcagatc tgggacacag
      661 ccggtcaaga acggttccag tctcttggtg tggccttcta cagaggtgca gattgctgtg
      721 ttctggtgtt tgatgtgact gcccccaaca ctttcaaaac cctcgacagc tggagagacg
      781 agtttctcat ccaggccagc ccccgggatc ccgagaactt ccctttt
//
