LOCUS       sequence-123184-         670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint universal donor for SpyTag fusion tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-SpyTag
FEATURES             Location/Qualifiers
     source          1..670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(111..128)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(111..127)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(120..142)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        337..441
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttgtaggcgt ccaccatcac gatgtgggcg gatccccctc cactgccacc agaccccccg
       61 ctttttgggt actggcccgc tagctatcat taggtaccga gctcgaattc actggccgtc
      121 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      181 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      241 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
      301 tgcggtattt cacaccgcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      361 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
      421 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
      481 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
      541 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
      601 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
      661 ctatgtggcg
//
