LOCUS       sequence-123242-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOPO construct expressing CloverCP for generation of GMAP-compatible
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: TOPO CloverCP
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     244..266
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     258..275
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     259..275
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     282..301
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        282..300
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(429..548)
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     CDS             complement(552..599)
                     /codon_start=1
                     /product="non-ORF yeast peptide conferring 
                     ubiquitin-dependent degradation"
                     /label=hCL1
                     /note="human codon-optimized"
                     /translation="ACKNWFSSLSHFVIHL"
     promoter        608..626
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     608..625
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(684..705)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcagacgtgc actggccagg gggatcacca tccgtcgccc cggcgtgtca ataatatcac
       61 tctgtacatc cacaaacaga cgataacggc tctctctttt ataggtgtaa accttaaact
      121 gccgtacgta taggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      181 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      241 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg
      301 gcgaattggg ccctctagat gcatgctcga gcggccgcca gtgtgatgga tatctgcaga
      361 attccagcac actggcggcc gttactagtg gatccagagt aattcaaccc caaacaacaa
      421 cgtttttaga cgttgatcct ggcgctggcg caagcagcag ggtgtctatc catgccgctc
      481 tcctgggcgc agctcatggg cagggtgccg gcggcctgct cctccacctc gggagggaag
      541 ccgtggctgt taaggtggat cacaaagtgg cttaagctac tgaaccagtt cttgcaagca
      601 gaattgcatt taggtgacac tatagaatag ggccctctag acttgtacag ctcgtccatg
      661 ccatgtgtaa tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg
      721 ttggggtctt tgctcagggc ggactgatgg ctcaggtagt ggttgtcggg cagcagcacg
      781 gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc
      841 tcaacgttgt ggcggatctt gaagttagcc ttgatgccgt tcttctgctt gtcggccgtg
      901 atatagacgt tgtggctgtt gaagttgtac tccagcttgt gccccaggat gttgccgtcc
      961 tccttgaagt cgatgccctt cagctcgatg cggttc
//
