LOCUS       sequence-123288-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retrovirus expressing GFP driven by UBC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: MSCV Puro-UBC:GFP
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(103..615,616..618,619..621))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     296..315
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     555..576
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(815..834)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 ggcggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg
       61 ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat
      121 cttgaagttc gccttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct
      181 gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc
      241 cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
      301 gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc
      361 ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc
      421 gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat
      481 gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccag acacgctgaa
      541 cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag
      601 ctcctcgccc ttgctcacca tggtccgctt aagctgtagc gctaatgtcg ggtcctagcg
      661 cgttagcttc gtctaacaaa aaagccaaaa acggccagaa tttagcggac aatttactag
      721 tctaacactg aaaattacat attgacccaa atgattacat ttcaaaaggt gcctaaaaaa
      781 cttcacaaaa cacactcgcc aaccccgagc gcatagttca aaaccggagc ttcagctact
      841 taagaagata ggtacataaa accgaccaaa gaaactgacg cctcacttat ccctcccctc
      901 accagaggtc cggcgcctgt cgattcagga gagcctaccc taggcccgaa ccctgcgtcc
      961 tgcgacggag aaaagcctac cgcacaccta ccg
//
