LOCUS       sequence-123296-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  redox-sensitive red fluorescent protein rxRFP1.3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Fan Y, Ai HW
  TITLE     Development of redox-sensitive red fluorescent proteins for imaging 
            redox dynamics in cellular compartments.
  JOURNAL   Anal Bioanal Chem. 2016 Jan 12.
  PUBMED    26758595
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Bioanal Chem. 2016 Jan 12."
COMMENT     Sequence Label: pBAD-rxRFP1.3
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             81..98
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             102..134
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(138..146,147..161)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 tgacgctttt ttcgcactct ctactgtttc tccatacccg ttttttgggc taacaggagg
       61 aattaaccat ggggggttct catcatcatc atcatcatgg tatggctagc atgactggtg
      121 gacagcaaat gggtcgggat ctgtacgacg atgacgataa ggatccgagc tcgagcgagc
      181 ttagttgcgc tataggtcgg ctgagctcac ccgtggtttc cgagcggatg taccccgagg
      241 acggcgccct gaagagcgag atcaagaagg ggctgaggct gaaggacggc ggccactacg
      301 ccgccgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
      361 tcgtcgacat caagttggac atcgtgtccc acaacgagga ctacaccatc gtggaacagt
      421 gcgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagggaggta
      481 caggcgggag tctggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
      541 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
      601 agggcgaggg ccgcccctac gaggcctttc agaccgctaa gctgaaggtg accaagggtg
      661 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
      721 acattaagca cccagccgac atccccgact acttcaagct gtccttcccc gagggcttca
      781 ggtgggagcg cgtgatgaac ttcgaggacg gcggcattat tcacgttaac caggactcct
//
