LOCUS       sequence-123330-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Histon2B-targetted Kohinoor for mammalian cell expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Tiwari DK, Arai Y, Yamanaka M, Matsuda T, Agetsuma M, Nakano M, 
            Fujita K, Nagai T
  TITLE     A fast- and positively photoswitchable fluorescent protein for 
            ultralow-laser-power RESOLFT nanoscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):515-8. doi: 10.1038/nmeth.3362. Epub 
            2015 Apr 20.
  PUBMED    25894946
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3362"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "515-8"
COMMENT     Sequence Label: Kohinoor-H2B/pcDNA3
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             102..773
                     /codon_start=1
                     /product="fast-switching photoswitchable fluorescent 
                     protein useful for fluorescence nanoscopy (Tiwari et al., 
                     2015)"
                     /label=Kohinoor
                     /translation="MSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKVK
                     EGGPLPFAYDILTMAFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMIYEDGGICIAT
                     NDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKSDGNYALSL
                     EGGGHYRCDSKTTYKAKKVVQLPDYHDVVHHIEIKSHDRDYSNVNLHEHAEAHSGLPRQ
                     AK"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg taccgagctc ggatccccac catgagtgtg attaaaccag
      121 acatgaagat caagctgcgt atggaaggcg ctgtaaatgg acacccgttc gcgattgaag
      181 gagttggcct tgggaagcct ttcgagggaa aacagagtat ggaccttaaa gtcaaagaag
      241 gcggacctct gcctttcgcc tatgacatct tgacaatggc cttctgttac ggcaacaggg
      301 tattcgccaa atacccagaa aatatagtag attatttcaa gcagtcgttt cctgagggct
      361 actcttggga acgaagcatg atttacgaag acgggggcat ttgtatcgcg acaaacgaca
      421 taaccctgga tggtgactgt tatatctatg aaattcgatt tgatggtgtg aactttcctg
      481 ccaatggtcc agttatgcag aagaggactg tgaaatggga gccatccact gagaaattgt
      541 atgtgcgtga tggagtgctg aagagcgatg gcaactacgc tctgtcgctt gaaggaggtg
      601 gccactaccg atgtgactcc aaaactactt ataaagctaa gaaggttgtc cagttgccag
      661 actatcacga tgtggtccac cacattgaga ttaaaagcca cgacagagat tacagtaatg
      721 ttaatctgca tgagcatgcc gaagcgcatt ctgggctgcc gaggcaggcc aaggaattca
      781 tgccg
//
