LOCUS       sequence-123473-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral backbone for use with GMAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: RV 2-5
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             74..490
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     420..442
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     458..474
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 tctgctgcag cgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
       61 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
      121 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
      181 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
      241 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
      301 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
      361 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
      421 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
      481 tctaggcgcc ggaattagat ctctaactcg aacgctagct gtgcgatcgt ttaaacgttg
      541 ttgtttgggg ttgaattact ctatcgataa aataaaagat tttatttagt ctccagaaaa
      601 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
      661 gcaaggcatg gaaaatacat aactgagaat agagaagttc agatcaaggt taggaacaga
      721 gagacagcag aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
      781 gggccaagaa cagatggtcc ccagatgcgg tcccgccctc agcagtttct agagaaccat
      841 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc
      901 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc
      961 ccacaacccc tcactcggcg cgccagtcct
//
