LOCUS       sequence-12349-e        9260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9260)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 9260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pBPHLWL-GAL80
FEATURES             Location/Qualifiers
     source          1..9260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    689..758
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     949..965
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1086..2393
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL80"
                     /product="transcriptional repressor that inhibits GAL4"
                     /label=GAL80
                     /note="GAL80 binds to and inhibits the transcriptional 
                     activation domain of GAL4."
                     /translation="MDYNKRSSVSTVPNAAPIRVGFVGLNAAKGWAIKTHYPAILQLSS
                     QFQITALYSPKIETSIATIQRLKLSNATAFPTLESFASSSTIDMIVIAIQVASHYEVVM
                     PLLEFSKNNPNLKYLFVEWALACSLDQAESIYKAAAERGVQTIISLQGRKSPYILRAKE
                     LISQGYIGDINSIEIAGNGGWYGYERPVKSPKYIYEIGNGVDLVTTTFGHTIDILQYMT
                     SSYFSRINAMVFNNIPEQELIDERGNRLGQRVPKTVPDHLLFQGTLLNGNVPVSCSFKG
                     GKPTKKFTKNLVIDIHGTKGDLKLEGDAGFAEISNLVLYYSGTRANDFPLANGQQAPLD
                     PGYDAGKEIMEVYHLRNYNAIVGNIHRLYQSISDFHFNTKKIPELPSQFVMQGFDFEGF
                     PTLMDALILHRLIESVYKSNMMGSTLNVSNISHYSL"
     protein_bind    complement(2680..2713)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2898..2916)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(3368..3439,3841..4114,4189..4843,4905..5220,5424..5555,
                     5626..6240)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    complement(7014..7047)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7072..7092)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7072..7090)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7111..7127)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7111..7127)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7124..7146)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7135..7151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7159..7165,7166..7183,7184..7189))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7204..7225
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7342..7359)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7513..8101)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7593..7612)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8272..9063,9064..9132))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8895..8914
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9133..9237)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gacgatgtag gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc
      721 ccttgggctc cccgggcgcg tactccacct cacccatctg gtccatcatg atgaacgggt
      781 cgaggtggcg gtagttgatc ccggcgaacg cgcggcgcac cgggaagccc tcgccctcga
      841 aaccgctggg cgcggtggtc acggtgagca cgggacgtgc gacggcgtcg gcgggtgcgg
      901 atacgcgggg cagcgtcagc gggttctcga cggtcacggc gggcatgtcg aggtcgacgg
      961 tatcgataag cttaccgaag tatacactta aattcagtgc acgtttgctt gttgagagga
     1021 aaggttgtgt gcggacgaat ttttttttga aaacattaac ccttacgtgg atccgcggcc
     1081 gcaacatgga ctacaacaag agatcttcgg tctcaaccgt gcctaatgca gctcccataa
     1141 gagtcggatt cgtcggtctc aacgcagcca aaggatgggc aatcaagaca cattaccccg
     1201 ccatactgca actatcgtca caatttcaaa tcactgcctt atacagtcca aaaattgaga
     1261 cttctattgc caccattcag cgtctaaaat tgagtaatgc cactgctttt cccactttag
     1321 agtcatttgc atcatcttcc actatagata tgatagtgat agctatccaa gtggccagcc
     1381 attatgaagt tgttatgcct ctcttggaat tctccaaaaa taatccgaac ctcaagtatc
     1441 ttttcgtaga atgggccctt gcatgttcac tagatcaagc cgaatccatt tataaggctg
     1501 ctgctgaacg tggggttcaa accatcatct ctttacaagg tcgtaaatca ccatatattt
     1561 tgagagcaaa agaattaata tctcaaggct atatcggcga cattaattcg atcgagattg
     1621 ctggaaatgg cggttggtac ggctacgaaa ggcctgttaa atcaccaaaa tacatctatg
     1681 aaatcgggaa cggtgtagat ctggtaacca caacatttgg tcacacaatc gatattttac
     1741 aatacatgac aagttcgtac ttttccagga taaatgcaat ggttttcaat aatattccag
     1801 agcaagagct gatagatgag cgtggtaacc gattgggcca gcgagtccca aagacagtac
     1861 cggatcatct tttattccaa ggcacattgt taaatggcaa tgttccagtg tcatgcagtt
     1921 tcaaaggtgg caaacctacc aaaaaattta ccaaaaattt ggtcattgac attcacggta
     1981 ccaagggaga tttgaaactt gaaggcgatg ccggcttcgc agaaatttca aatctggtcc
     2041 tttactacag tggaactaga gcaaacgact tcccgctagc caatggacaa caagctcctt
     2101 tagacccggg gtatgatgca ggtaaagaaa tcatggaagt atatcattta cgaaattata
     2161 atgccattgt gggtaatatt catcgactgt atcaatctat ctctgacttc cacttcaata
     2221 caaagaaaat tcctgaatta ccctcacaat ttgtaatgca aggtttcgat ttcgaaggct
     2281 ttcccacctt gatggatgct ctgatattac acaggttaat cgagagcgtt tataaaagta
     2341 acatgatggg ctccacatta aacgttagca atatctcgca ttatagttta taatctagat
     2401 cgataccgtc gactaaagcc aaatagaaaa ttattcagtt cctggcttaa gtttttaaaa
     2461 gtgatattat ttatttggtt gtaaccaacc aaaagaatgt aaataactaa tacataatta
     2521 tgttagtttt aagttagcaa caaattgatt ttagctatat tagctacttg gttaataaat
     2581 agaatatatt tatttaaaga taattgcgtt tttattgtca gggagtgagt ttgcttaaaa
     2641 actcgtttag atccactagc ttaattaagc atgcactaga taacttcgta tagcatacat
     2701 tatacgaagt tatagcttct gatggaatta gaacttggca aaacaatact gagaatgaag
     2761 tgtatgtgga acagaggctg ctgatctcgt tcttcaggct atgaaactga cacatttgga
     2821 aaccacagta cttagaacca caaagtggga atcaagagaa aaacaatgat cccacgagag
     2881 atcgaattct agtatgtatg taagttaata aaacccattt ttgcggaaag tagataaaaa
     2941 aaacattttt tttttttact gcactggata tcattgaact tatctgatca gttttaaatt
     3001 tacttcgatc caagggtatt tgatgtacca ggttctttcg attacctctc actcaaaatg
     3061 acattccact caaagtcagc gctgtttgcc tccttctctg tccacagaaa tatcgccgtc
     3121 tctttcgccg ctgcgtccgc tatctctttc gccaccgttt gtagcgttac gtagcgtcaa
     3181 tgtccgcctt cagttgcatt ttgtcagcgg tttcgtgacg aagctccaag cggtttacgc
     3241 catcaattaa acacaaagtg ctgtgccaaa actcctctcg cttcttattt ttgtttgttt
     3301 tttgagtgat tggggtggtg attggttttg ggtgggtaag caggggaaag tgtgaaaaat
     3361 cccggcaatg ggccaagagg atcaggagct attaattcgc ggaggcagca aacacccatc
     3421 tgccgagcat ctgaacaatg tgagtagtac atgtgcatac atcttaagtt cacttgatct
     3481 ataggaactg cgattgcaac atcaaattgt ctgcggcgtg agaactgcga cccacaaaaa
     3541 tcccaaaccg caattgcaca aacaaatagt gacacgaaac agattattct ggtagctgtt
     3601 ctcgctatat aagacaattt ttgagatcat atcatgatca agacatctaa aggcattcat
     3661 tttcgactat attctttttt acaaaaaata taacaaccag atattttaag ctgatcctag
     3721 atgcacaaaa aataaataaa agtataaacc tacttcgtag gatacttcgg ggtacttttt
     3781 gttcggggtt agatgagcat aacgcttgta gttgatattt gagatcccct atcattgcag
     3841 ggtgacagcg gagcggcttc gcagagctgc attaaccagg gcttcgggca ggccaaaaac
     3901 tacggcacgc tccggccacc cagtccgccg gaggactccg gttcagggag cggccaacta
     3961 gccgagaacc tcacctatgc ctggcacaat atggacatct ttggggcggt caatcagccg
     4021 ggctccggat ggcggcagct ggtcaaccgg acacgcggac tattctgcaa cgagcgacac
     4081 ataccggcgc ccaggaaaca tttgctcaag aacggtgagt ttctattcgc agtcggctga
     4141 tctgtgtgaa atcttaataa agggtccaat taccaatttg aaactcagtt tgcggcgtgg
     4201 cctatccggg cgaacttttg gccgtgatgg gcagttccgg tgccggaaag acgaccctgc
     4261 tgaatgccct tgcctttcga tcgccgcagg gcatccaagt atcgccatcc gggatgcgac
     4321 tgctcaatgg ccaacctgtg gacgccaagg agatgcaggc caggtgcgcc tatgtccagc
     4381 aggatgacct ctttatcggc tccctaacgg ccagggaaca cctgattttc caagccatgg
     4441 tgcggatgcc acgacatctg acctatcggc agcgagtggc ccgcgtggat caggtgatcc
     4501 aggagctttc gctcagcaaa tgtcagcaca cgatcatcgg tgtgcccggc agggtgaaag
     4561 gtctgtccgg cggagaaagg aagcgtctgg cattcgcctc cgaggctcta accgatccgc
     4621 cgcttctgat ctgcgatgag cccacctccg gactggactc ctttaccgcc cacagcgtcg
     4681 tccaggtgct gaagaagctg tcgcagaagg gcaagaccgt catcctgacc attcatcagc
     4741 cgtcttccga gctgtttgag ctctttgaca agatccttct gatggccgag ggcagggtag
     4801 ctttcttggg cactcccagc gaagccgtcg acttcttttc ctagtgagtt cgatgtgttt
     4861 attaagggta tctagtatta cataacatct caactcctat ccagcgtggg tgcccagtgt
     4921 cctaccaact acaatccggc ggacttttac gtacaggtgt tggccgttgt gcccggacgg
     4981 gagatcgagt cccgtgatcg gatcgccaag atatgcgaca attttgccat tagcaaagta
     5041 gcccgggata tggagcagtt gttggccacc aaaaatctgg agaagccact ggagcagccg
     5101 gagaatgggt acacctacaa ggccacctgg ttcatgcagt tccgggcggt cctgtggcga
     5161 tcctggctgt cggtgctcaa ggaaccactc ctcgtaaaag tgcgacttat tcagacaacg
     5221 gtgagtggtt ccagtggaaa caaatgatat aacgcttaca attcttggaa acaaattcgc
     5281 tagattttag atagaattgc ctgattccac acccttctta gtttttttca atgagatgta
     5341 tagtttatag ttttgcagaa gataaataaa tttcatttaa ctcgcgaata ttaatgagat
     5401 gcgagtaaca ttttaatttg cagatggttg ccatcttgat tggcctcatc tttttgggcc
     5461 aacaactcac gcaagtgggt gtgatgaata tcaacggagc catcttcctc ttcctgacca
     5521 acatgacctt tcaaaacgtc tttgccacga taaatgtaag tcatgtttag aatacatttg
     5581 catttcaata atttactaac tttctaatga atcgattcga tttaggtgtt cacctcagag
     5641 ctgccagttt ttatgaggga ggcccgaagt cgactttatc gctgtgacac atactttctg
     5701 ggcaaaacga ttgccgaatt gccgcttttt ctcacagtgc cactggtctt cacggcgatt
     5761 gcctatccga tgatcggact gcgggccgga gtgctgcact tcttcaactg cctggcgctg
     5821 gtcactctgg tggccaatgt gtcaacgtcc ttcggatatc taatatcctg cgccagctcc
     5881 tcgacctcga tggcgctgtc tgtgggtccg ccggttatca taccattcct gctctttggc
     5941 ggcttcttct tgaactcggg ctcggtgcca gtatacctca aatggttgtc gtacctctca
     6001 tggttccgtt acgccaacga gggtctgctg attaaccaat gggcggacgt ggagccgggc
     6061 gaaattagct gcacatcgtc gaacaccacg tgccccagtt cgggcaaggt catcctggag
     6121 acgcttaact tctccgccgc cgatctgccg ctggactacg tgggtctggc cattctcatc
     6181 gtgagcttcc gggtgctcgc atatctggct ctaagacttc gggcccgacg caaggagtag
     6241 ccgacatata tccgaaataa ctgcttgttt ttttttttta ccattattac catcgtgttt
     6301 actgtttatt gccccctcaa aaagctaatg taattatatt tgtgccaata aaaacaagat
     6361 atgacctata gaatacaagt atttcccctt cgaacatccc cacaagtaga ctttggattt
     6421 gtcttctaac caaaagactt acacacctgc ataccttaca tcaaaaactc gtttatcgct
     6481 acataaaaca ccgggatata ttttttatat acatactttt caaatcgcgc gccctcttca
     6541 taattcacct ccaccacacc acgtttcgta gttgctcttt cgctgtctcc cacccgctct
     6601 ccgcaacaca ttcacctttt gttcgacgac cttggagcga ctgtcgttag ttccgcgcga
     6661 ttcggttcgc tcaaatggtt ccgagtggtt catttcgtct caatagaaat tagtaataaa
     6721 tatttgtatg tacaatttat ttgctccaat atatttgtat atatttccct cacagctata
     6781 tttattctaa tttaatatta tgacttttta aggtaatttt ttgtgacctg ttcggagtga
     6841 ttagcgttac aatttgaact gaaagtgaca tccagtgttt gttccttgtg tagatgcatc
     6901 tcaaaaaaat ggtgggcata atagtgttgt ttatatatat caaaaataac aactataata
     6961 ataagaatac atttaattta gaaaatgctt ggatttcact ggaactagaa ttcataactt
     7021 cgtatagcat acattatacg aagttatgga tctgagctcc agcttttgtt ccctttagtg
     7081 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7141 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7201 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7261 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7321 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7381 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7441 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7501 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7561 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7621 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7681 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7741 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7801 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7861 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7921 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     7981 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8041 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8101 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8161 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8221 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8281 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8341 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8401 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8461 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8521 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8581 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8641 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8701 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8761 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8821 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8881 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8941 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9001 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9061 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9121 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9181 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9241 atttccccga aaagtgccac
//
