LOCUS       sequence-123537-         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Cas9 with His, MBP, and Strep tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  TITLE     Gerard Cagney unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: NM-01
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             34..56
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             64..96
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             142..159
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gggggcggga taatttcccc ttctaggaat aattttgttt aactttaaga aggagatata
       61 catatggcta gcatgactgg tggacagcaa atgggtcgcg gatccgaatt cgagctccgt
      121 cgacaagctt gcggccgcat gcatcaccat caccatcacc ccatgaaaat cgaagaaggt
      181 aaactggtaa tctggattaa cggcgataaa ggctataacg gtctcgctga agtcggtaag
      241 aaattcgaga aagataccgg aattaaagtc accgttgagc atccggataa actggaagag
      301 aaattcccac aggttgcggc aactggcgat ggccctgaca ttatcttctg ggcacacgac
      361 cgctttggtg gctacgctca atctggcctg ttggctgaaa tcaccccgga caaagcgttc
      421 caggacaagc tgtatccgtt tacctgggat gccgtacgtt acaacggcaa gctgattgct
      481 tacccgatcg ctgttgaagc gttatcgctg atttataaca aagatctgct gccgaacccg
      541 ccaaaaacct gggaagagat cccggcgctg gataaagaac tgaaagcgaa aggtaagagc
      601 gcgctgatgt tcaacctgca agaaccgtac ttcacctggc cgctgattgc tgctgacggg
      661 ggttatgcgt tcaagtatga aaacggcaag tacgacatta aagacgtggg gcgtggataa
      721 acgctgggcg cgaaagcggg tctgaccttc ctggttgacc ctggatt
//
