LOCUS       sequence-123538-         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Cas9 with His, MBP, and Strep tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  TITLE     Gerard Cagney unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: NM-01
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             628..651
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             661..678
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aaaagagtta ctagggatca aaaattatgg aaaagaagtt cctttgaaaa aaatccgatt
       61 gactttttag aagctaaagg atataaggaa gttaaaaaag acttaatcat taaactacct
      121 aaatatagtc tttttgagtt agaaaacggt cgtaaacgga tgctggctag tgccggagaa
      181 ttacaaaaag gaaatgagct ggctctgcca agcaaatatg tgaatttttt atatttagct
      241 agtcattatg aaaagttgaa gggtagtcca gaagataacg aacaaaaaca attgtttgtg
      301 gagcagcata agcattattt agatgagatt attgagcaaa tcagtgaatt ttctaagcgt
      361 gttattttag cagatgccaa tttagataaa gttcttagtg catataacaa acatagagac
      421 aaaccaatac gtgaacaagc agaaaatatt attcatttat ttacgttgac gaatcttgga
      481 gctcccgctg cttttaaata ttttgataca acaattgatc gtaaacgata tacgtctaca
      541 aaagaagttt tagatgccac tcttatccat caatccatca ctggtcttta tgaaacacgc
      601 attgatttga gtcagctagg aggtgactgg agccacccgc agttcgaaaa atgactcgag
      661 caccaccacc accaccactg agatccggct gctaacaaag cccgaaagaa gctacgccca
//
