LOCUS       sequence-12354-e        8757 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8757)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 8757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pXN-FBLWLF
FEATURES             Location/Qualifiers
     source          1..8757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(25..55,56..609)
                     /label=P element 5' end
     protein_bind    complement(623..656)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    701..770
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     960..976
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     961..977
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1082..1098)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1082..1098)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(1135..1168)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(1353..1371)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1823..1894,2296..2569,2644..3298,3360..3675,3879..4010,
                     4081..4695)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    complement(5469..5502)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(5526..5559)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    join(5789..5990,5991..6021)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(6558..6577)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6677..6699
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6737..6755)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6823..6927
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6928..6996,6997..7788)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7146..7165)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7959..8547
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8448..8467
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgctggact acgtgggtct ggcccatgat gaaataacat aaggtggtcc cgtcgatagc
       61 cgaagcttac cgaagtatac acttaaattc agtgcacgtt tgcttgttga gaggaaaggt
      121 tgtgtgcgga caattttttt ttgaaaacat taacccttac gtggaataaa aaaaaatgaa
      181 atattgcaaa ttttgctgca aagctgtgac tggagtaaaa ttaattcacg tgccgaagtg
      241 tgctattaag agaaaattgt gggagcagag ccttgggtgc agccttggtg aaaactccca
      301 aatttgtgat acccacttta atgattcgca gtggaaggct gcacctgcaa aaggtcagac
      361 atttaaaagg aggcgactca acgcagatgc cgtacctagt aaagtgatag agcctgaacc
      421 agaaaagata aaagaaggct ataccagtgg gagtacacaa acagagtaag tttgaatagt
      481 aaaaaaaatc atttatgtaa acaataacgt gactgtgcgt taggtcctgt tcattgttta
      541 atgaaaataa gagcttgagg gaaaaaattc gtactttgga gtacgaaatg cgtcgtttag
      601 agcagcagcc gaattcggta ccgaagttcc tatactttct agagaatagg aacttcggcg
      661 cgccagtacc gggccccccc tcgacgatgt aggtcacggt ctcgaagccg cggtgcgggt
      721 gccagggcgt gcccttgggc tccccgggcg cgtactccac ctcacccatc tggtccatca
      781 tgatgaacgg gtcgaggtgg cggtagttga tcccggcgaa cgcgcggcgc accgggaagc
      841 cctcgccctc gaaaccgctg ggcgcggtgg tcacggtgag cacgggacgt gcgacggcgt
      901 cggcgggtgc ggatacgcgg ggcagcgtca gcgggttctc gacggtcacg gcgggcatgt
      961 cgaggtcgac ggtatcgata agcttaccga agtatacact taaattcagt gcacgtttgc
     1021 ttgttgagag gaaaggttgt gtgcggacga attttttttt gaaaacatta acccttacgt
     1081 ggatccacta gttctagagc ggccgctcga gttaattaag catgcctagg ctagataact
     1141 tcgtatagca tacattatac gaagttatag cttctgatgg aattagaact tggcaaaaca
     1201 atactgagaa tgaagtgtat gtggaacaga ggctgctgat ctcgttcttc aggctatgaa
     1261 actgacacat ttggaaacca cagtacttag aaccacaaag tgggaatcaa gagaaaaaca
     1321 atgatcccac gagagatcga attctagtat gtatgtaagt taataaaacc catttttgcg
     1381 gaaagtagat aaaaaaaaca tttttttttt ttactgcact ggatatcatt gaacttatct
     1441 gatcagtttt aaatttactt cgatccaagg gtatttgatg taccaggttc tttcgattac
     1501 ctctcactca aaatgacatt ccactcaaag tcagcgctgt ttgcctcctt ctctgtccac
     1561 agaaatatcg ccgtctcttt cgccgctgcg tccgctatct ctttcgccac cgtttgtagc
     1621 gttacgtagc gtcaatgtcc gccttcagtt gcattttgtc agcggtttcg tgacgaagct
     1681 ccaagcggtt tacgccatca attaaacaca aagtgctgtg ccaaaactcc tctcgcttct
     1741 tatttttgtt tgttttttga gtgattgggg tggtgattgg ttttgggtgg gtaagcaggg
     1801 gaaagtgtga aaaatcccgg caatgggcca agaggatcag gagctattaa ttcgcggagg
     1861 cagcaaacac ccatctgccg agcatctgaa caatgtgagt agtacatgtg catacatctt
     1921 aagttcactt gatctatagg aactgcgatt gcaacatcaa attgtctgcg gcgtgagaac
     1981 tgcgacccac aaaaatccca aaccgcaatt gcacaaacaa atagtgacac gaaacagatt
     2041 attctggtag ctgttctcgc tatataagac aatttttgag atcatatcat gatcaagaca
     2101 tctaaaggca ttcattttcg actatattct tttttacaaa aaatataaca accagatatt
     2161 ttaagctgat cctagatgca caaaaaataa ataaaagtat aaacctactt cgtaggatac
     2221 ttcggggtac tttttgttcg gggttagatg agcataacgc ttgtagttga tatttgagat
     2281 cccctatcat tgcagggtga cagcggagcg gcttcgcaga gctgcattaa ccagggcttc
     2341 gggcaggcca aaaactacgg cacgctccgg ccacccagtc cgccggagga ctccggttca
     2401 gggagcggcc aactagccga gaacctcacc tatgcctggc acaatatgga catctttggg
     2461 gcggtcaatc agccgggctc cggatggcgg cagctggtca accggacacg cggactattc
     2521 tgcaacgagc gacacatacc ggcgcccagg aaacatttgc tcaagaacgg tgagtttcta
     2581 ttcgcagtcg gctgatctgt gtgaaatctt aataaagggt ccaattacca atttgaaact
     2641 cagtttgcgg cgtggcctat ccgggcgaac ttttggccgt gatgggcagt tccggtgccg
     2701 gaaagacgac cctgctgaat gcccttgcct ttcgatcgcc gcagggcatc caagtatcgc
     2761 catccgggat gcgactgctc aatggccaac ctgtggacgc caaggagatg caggccaggt
     2821 gcgcctatgt ccagcaggat gacctcttta tcggctccct aacggccagg gaacacctga
     2881 ttttccaagc catggtgcgg atgccacgac atctgaccta tcggcagcga gtggcccgcg
     2941 tggatcaggt gatccaggag ctttcgctca gcaaatgtca gcacacgatc atcggtgtgc
     3001 ccggcagggt gaaaggtctg tccggcggag aaaggaagcg tctggcattc gcctccgagg
     3061 ctctaaccga tccgccgctt ctgatctgcg atgagcccac ctccggactg gactccttta
     3121 ccgcccacag cgtcgtccag gtgctgaaga agctgtcgca gaagggcaag accgtcatcc
     3181 tgaccattca tcagccgtct tccgagctgt ttgagctctt tgacaagatc cttctgatgg
     3241 ccgagggcag ggtagctttc ttgggcactc ccagcgaagc cgtcgacttc ttttcctagt
     3301 gagttcgatg tgtttattaa gggtatctag tattacataa catctcaact cctatccagc
     3361 gtgggtgccc agtgtcctac caactacaat ccggcggact tttacgtaca ggtgttggcc
     3421 gttgtgcccg gacgggagat cgagtcccgt gatcggatcg ccaagatatg cgacaatttt
     3481 gccattagca aagtagcccg ggatatggag cagttgttgg ccaccaaaaa tctggagaag
     3541 ccactggagc agccggagaa tgggtacacc tacaaggcca cctggttcat gcagttccgg
     3601 gcggtcctgt ggcgatcctg gctgtcggtg ctcaaggaac cactcctcgt aaaagtgcga
     3661 cttattcaga caacggtgag tggttccagt ggaaacaaat gatataacgc ttacaattct
     3721 tggaaacaaa ttcgctagat tttagataga attgcctgat tccacaccct tcttagtttt
     3781 tttcaatgag atgtatagtt tatagttttg cagaagataa ataaatttca tttaactcgc
     3841 gaatattaat gagatgcgag taacatttta atttgcagat ggttgccatc ttgattggcc
     3901 tcatcttttt gggccaacaa ctcacgcaag tgggtgtgat gaatatcaac ggagccatct
     3961 tcctcttcct gaccaacatg acctttcaaa acgtctttgc cacgataaat gtaagtcatg
     4021 tttagaatac atttgcattt caataattta ctaactttct aatgaatcga ttcgatttag
     4081 gtgttcacct cagagctgcc agtttttatg agggaggccc gaagtcgact ttatcgctgt
     4141 gacacatact ttctgggcaa aacgattgcc gaattgccgc tttttctcac agtgccactg
     4201 gtcttcacgg cgattgccta tccgatgatc ggactgcggg ccggagtgct gcacttcttc
     4261 aactgcctgg cgctggtcac tctggtggcc aatgtgtcaa cgtccttcgg atatctaata
     4321 tcctgcgcca gctcctcgac ctcgatggcg ctgtctgtgg gtccgccggt tatcatacca
     4381 ttcctgctct ttggcggctt cttcttgaac tcgggctcgg tgccagtata cctcaaatgg
     4441 ttgtcgtacc tctcatggtt ccgttacgcc aacgagggtc tgctgattaa ccaatgggcg
     4501 gacgtggagc cgggcgaaat tagctgcaca tcgtcgaaca ccacgtgccc cagttcgggc
     4561 aaggtcatcc tggagacgct taacttctcc gccgccgatc tgccgctgga ctacgtgggt
     4621 ctggccattc tcatcgtgag cttccgggtg ctcgcatatc tggctctaag acttcgggcc
     4681 cgacgcaagg agtagccgac atatatccga aataactgct tgtttttttt ttttaccatt
     4741 attaccatcg tgtttactgt ttattgcccc ctcaaaaagc taatgtaatt atatttgtgc
     4801 caataaaaac aagatatgac ctatagaata caagtatttc cccttcgaac atccccacaa
     4861 gtagactttg gatttgtctt ctaaccaaaa gacttacaca cctgcatacc ttacatcaaa
     4921 aactcgttta tcgctacata aaacaccggg atatattttt tatatacata cttttcaaat
     4981 cgcgcgccct cttcataatt cacctccacc acaccacgtt tcgtagttgc tctttcgctg
     5041 tctcccaccc gctctccgca acacattcac cttttgttcg acgaccttgg agcgactgtc
     5101 gttagttccg cgcgattcgg ttcgctcaaa tggttccgag tggttcattt cgtctcaata
     5161 gaaattagta ataaatattt gtatgtacaa tttatttgct ccaatatatt tgtatatatt
     5221 tccctcacag ctatatttat tctaatttaa tattatgact ttttaaggta attttttgtg
     5281 acctgttcgg agtgattagc gttacaattt gaactgaaag tgacatccag tgtttgttcc
     5341 ttgtgtagat gcatctcaaa aaaatggtgg gcataatagt gttgtttata tatatcaaaa
     5401 ataacaacta taataataag aatacattta atttagaaaa tgcttggatt tcactggaac
     5461 tagaattcat aacttcgtat agcatacatt atacgaagtt atggatctgg ccgcgctctt
     5521 ccgctgaagt tcctatactt tctagagaat aggaacttcc tcgacggtag cggtccggtt
     5581 gttttcgtgc tcatcgcgag tacgtattta tttttaaaaa ataataaacg cggcaaacac
     5641 aatcacacaa atgtgcaggc acagtgggac aaagtgcgct aaatttggat atgtaataaa
     5701 tccaaaacca aaaagaaaac aaaaaacggt aaaatatttt actgttgtta aaattcgatc
     5761 attcattatt cgctgcatga attagctttg cgtactcgca aattattaaa aataaaactt
     5821 taaaaataat ttcgtctaat taatattatg agttaattca aaccccacgg acatgctaag
     5881 ggttaatcaa caatcatatc gctgtctcac tcagactcaa tacgacactc agaatactat
     5941 tcctttcact cgcacttatt gcaagcatac gttaagtgga tgtctcttgc cgacgggacc
     6001 accttatgtt atttcatcat ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat
     6061 atctggctct aagacttcgg gcccgacgca aggagtagcc gacatatatc cgaaataact
     6121 gcttgttttt ttttttacca ttattaccat cgtgtttact gtttattgcc ccctcaaaaa
     6181 gctaatgtaa ttatatttgt gccaataaaa acaagatatg acctatagaa tacaagtatt
     6241 tccccttcga acatccccac aagtagactt tggatttgtc ttctaaccaa aagacttaca
     6301 cacctgcata ccttacatca aaaactcgtt tatcgctaca taaaacaccg ggatatattt
     6361 tttatataca tacttttcaa atcgcgcgcc ctcttcataa ttcacctcca ccacaccacg
     6421 tttcgtagtt gctctttcgc tgtctcccac ccgctctccg caacacattc accttttgtt
     6481 cgacgacctt ggagcgactg tcgttagttc cgcgcgattc ggtgcggtat ttcacaccgc
     6541 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     6601 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     6661 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     6721 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     6781 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6841 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6901 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6961 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     7021 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     7081 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     7141 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     7201 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     7261 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     7321 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     7381 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     7441 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     7501 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     7561 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7621 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7681 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7741 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7801 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7861 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7921 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7981 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     8041 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     8101 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     8161 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     8221 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     8281 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     8341 cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     8401 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     8461 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     8521 tcgtcagggg ggcggagcct atggaaaaac gccttcttct tgaactcggg ctcggtgcca
     8581 gtatacctca aatggttgtc gtacctctca tggttccgtt acgccaacga gggtctgctg
     8641 attaaccaat gggcggacgt ggagccgggc gaaattagct gcacatcgtc gaacaccacg
     8701 tgccccagtt cgggcaaggt catcctggag acgcttaact tctccgccgc cgatctg
//
