LOCUS       sequence-123554-        1097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Lawson ND, Vogel AM, Weinstein BM
  TITLE     sonic hedgehog and vascular endothelial growth factor act upstream 
            of the Notch pathway during arterial endothelial differentiation.
  JOURNAL   Dev Cell. 2002 Jul . 3(1):127-36.
  PUBMED    12110173
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2002 Jul . 3(1):127-36."
COMMENT     Sequence Label: 35 pCR vegf 121
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(902..920)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(903..920)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(938..954)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(938..954)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(951..973)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    962..978
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1030..1051
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 actcactata gggcgaattg ggccctctag atgcatgctc gagcggccgc cagtgtgatg
       61 gatatctgca gaattcgccc tttatactcg agctgcactt agtgtttcgt ttaggatagt
      121 gtttgcagtt catccaagga atgttcttcc ttaggttttg aaagtgctgt gccgtttaaa
      181 aagtttttat ttaagttcct tccttcatgc agagttctgc ccggcgttcg ctcgatcatc
      241 atcttggctt ttcacatctg caagttcttt cgtttaactc aagttgtctg gacttgcatt
      301 gcatttgtgt gaatttgcag gagcatttac aggtgagggg gtcctgcaca tacaagcgct
      361 tccttctctc tgagcaaggc tcacagtggt tttctttctt tgctttgact tctgcctttg
      421 gcctgcattc acacttggtg tgttctgtga aactcagctg aaaattatgc tgcgatacgc
      481 gttgcttgac ccgcagcacc tccatagtga cgtttcgtgt ctctgtcggg acgcattcga
      541 gcgcctcatc attacagcat cctgcacagc gcatgagaac cacacaggac gggatgtacg
      601 tgtgctcgat ctcatcggga tactcctgga tgatgtctac cagcagctct cgggtcttgc
      661 acgcactctt tttatacaca tccatgaagg gaatcacatc atttttgctc tttccccctt
      721 ctttgggtat gtgggcagcc tttacagcag acagatggag gagagccgcg agaaataact
      781 gtatcaaata aacaaccaag ttcatgattg tggatcctat aaagggcgaa ttccagcaca
      841 ctggcggccg ttactagtgg atccgagctc ggtaccaagc tttgatgcat agcttgagta
      901 ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
      961 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataagt gtaaagcctg
     1021 gggtgcctaa tgagtgagct aactcacatt aatgcgttgc gctcactgcc cgctttccag
     1081 tcggaaaacc tgtcgtg
//
