LOCUS       sequence-123586-        1068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For in situ antisense probe, NotI w/ Sp6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Kobayashi I, Kobayashi-Sun J, Kim AD, Pouget C, Fujita N, Suda T, 
            Traver D
  TITLE     Jam1a-Jam2a interactions regulate haematopoietic stem cell fate 
            through Notch signalling.
  JOURNAL   Nature. 2014 Aug 21;512(7514):319-23. doi: 10.1038/nature13623. Epub
            2014 Aug 13.
  PUBMED    25119047
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13623"; journalName: "Nature"; date: "2014-08-21- 
            21"; volume: "512"; issue: "7514"; pages: "319-23"
COMMENT     Sequence Label: jam2a-pCRII
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(776..795)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(777..795)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(802..819)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(802..818)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(811..833)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ctatagaata ctcaagctat gcatcaagct tgggtaccga gctcggatcc actagtaacg
       61 gccgccagtg tgctggaatt cgcccttcag aaccacagct gagaccaccg ccgccacgtt
      121 caggtcatcg atctgcatgt gtgtgtgctg gcagctcttg gggggtccaa ctccattttt
      181 ggcctcacag tgatactgac ctgcatctga gcggctcacc gtctgaaaca tcagtacacc
      241 ggtgaactca ttgacggtgt aggtggcgtt gggatggcgg atgggcagtg cgcggttgtc
      301 tttgtaccag gtgtagacgg caggtgggat gctgtgtcgg tctctacagc gcagctccac
      361 ctgagagccc gtcagagcag agctgggcac gtcacatgag ggagtctgag gaggaaccag
      421 aactctcagt gtgacgttgg tttctcccag tgagatggag tcactcggag cgctgacctc
      481 acaacgatat tcgcctgcgt ccgcctgagt gaccctgcgt agccgcactg tcgctccttc
      541 aatatctgcg cgatcctgga acggccctac gaatcgttct ccatagtaga cgaaggacac
      601 gtctttttcc ttgtctttcc tcttccactc gatgcgaggg ttggtgtctt tctcagtctt
      661 gaactcgcag gagagctctg cgtctgagaa ctcgtgcacc tccactaagg gcgaattctg
      721 cagatatcca tcacactggc ggccgctcga gcatgcatct agagggccca attcgcccta
      781 tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
      841 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      901 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaaat ggcgaatgga
      961 cgccccctgt aagcggcgca ttaagcgcgg ccgggtgttg gtggttacgc gcagcgtgaa
     1021 ccgctacact tgccagcgcc tagcgccgcc tcctttcgct ttctttcc
//
