LOCUS       sequence-123656-       10941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Episomal plasmid encoding  5 gRNAS targeting human OCT4 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10941)
  AUTHORS   Balboa D, Weltner J, Eurola S, Trokovic R, Wartiovaara K, Otonkoski 
            T
  TITLE     Conditionally Stabilized dCas9 Activator for Controlling Gene 
            Expression in Human Cell Reprogramming and Differentiation.
  JOURNAL   Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 
            10.1016/j.stemcr.2015.08.001.
  PUBMED    26352799
REFERENCE   2  (bases 1 to 10941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2015.08"; journalName: "Stem Cell Reports"; date: 
            "2015-09-8- 8"; volume: "5"; issue: "3"; pages: "448-59"
COMMENT     Sequence Label: GG-EBNA-OCT4
FEATURES             Location/Qualifiers
     source          1..10941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(190..209)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     309..331
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(369..387)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        455..559
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(560..628,629..1420)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(778..797)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1591..2179
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2080..2099
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    2467..2488
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2503..2508,2509..2526,2527..2533)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2541..2557
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2546..2568
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2565..2581
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2600..2619
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2600..2618
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2685..2701
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2702..2950
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        2971..3046
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     3078..3094
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3095..3343
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        3365..3440
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(3452..3468)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3472..3488
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3489..3737
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        3759..3834
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     3866..3882
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3883..4131
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        4153..4228
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     4260..4276
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4277..4525
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        4547..4622
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    4780..4813
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4874..5371
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4909..4932)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5304..5323
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5411..6010
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5411..5430)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5907..5927
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6459..8384
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    8552..8585
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      8772..10561
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 gcactgcccc gcgaagaagg ggcagagatg tcgtagtcag gtttagttcg tccggggcgg
       61 ggcatcgatc ctctagtaag ggattttgcc gatttcggcc tattggttaa aaaatgagct
      121 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
      181 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
      241 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
      301 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
      361 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
      421 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      481 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      541 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      601 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      661 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
      721 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
      781 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
      841 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
      901 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
      961 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     1021 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     1081 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     1141 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     1201 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     1261 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     1321 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     1381 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     1441 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     1501 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1561 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1621 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1681 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1741 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1801 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1861 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1921 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1981 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2041 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2101 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2161 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2221 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2281 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2341 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2401 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     2461 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     2521 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     2581 ccatgattac gccaagctct aatacgactc actataggga gacaagcttg catgcctgca
     2641 ggtcgactct agaggatctc gagctcaagc ttcgaattcc ccctgtaaaa cgacggccag
     2701 tgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
     2761 gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
     2821 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
     2881 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
     2941 acgaaacacc ggggggagaa actgaggcga gttttagagc tagaaatagc aagttaaaat
     3001 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttcatggtc
     3061 atagctgttt cctcatggta aaacgacggc cagtgagggc ctatttccca tgattccttc
     3121 atatttgcat atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa
     3181 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc
     3241 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt
     3301 cgatttcttg gctttatata tcttgtggaa aggacgaaac accgggtggt ggcaatggtg
     3361 tctggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa
     3421 aagtggcacc gagtcggtgc tttttttcat ggtcatagct gtttcctgga cgtaaaacga
     3481 cggccagtga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg
     3541 ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt
     3601 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
     3661 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     3721 ggaaaggacg aaacaccgga cacaactggc gcccctccgt tttagagcta gaaatagcaa
     3781 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
     3841 tcatggtcat agctgtttcc tccaggtaaa acgacggcca gtgagggcct atttcccatg
     3901 attccttcat atttgcatat acgatacaag gctgttagag agataattgg aattaatttg
     3961 actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg
     4021 tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga
     4081 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac cgggcacagt
     4141 gccagaggtc tggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     4201 acttgaaaaa gtggcaccga gtcggtgctt tttttcatgg tcatagctgt ttccttgttg
     4261 taaaacgacg gccagtgagg gcctatttcc catgattcct tcatatttgc atatacgata
     4321 caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca
     4381 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
     4441 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
     4501 tatcttgtgg aaaggacgaa acaccgtctg tgggggacct gcactggttt tagagctaga
     4561 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
     4621 gctttttttc atggtcatag ctgtttccta acgagcggcc gcaggaaccc ctagtgatgg
     4681 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     4741 cccgacgccc aattccgatc atattcaata acccttaata taacttcgta taatgtatgc
     4801 tatacgaagt tattaggtct gaagaggagt ttacgtccag ccaagcttag gatctcgacc
     4861 tcgaaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
     4921 gcagccccgc tggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     4981 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     5041 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     5101 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     5161 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcagc
     5221 agctgggaag ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     5281 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     5341 gttctcctct tcctcatctc cgggcctttc gacctgcatc catctagatc tcgagcagct
     5401 gaagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     5461 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     5521 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     5581 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     5641 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     5701 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     5761 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     5821 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     5881 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     5941 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     6001 cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca
     6061 tgcatcgata agctgatcct cacaggccgc acccagcttt tcttccgttg ccccagtagc
     6121 atctctgtct ggtgaccttg aagaggaaga ggaggggtcc cgagaatccc catccctacc
     6181 gtccagcaaa aagggggacg aggaatttga ggcctggctt gaggctcagg acgcaaatct
     6241 tgaggatgtt cagcgggagt tttccgggct gcgagtaatt ggtgatgagg acgaggatgg
     6301 ttcggaggat ggggaatttt cagacctgga tctgtctgac agcgaccatg aaggggatga
     6361 gggtgggggg gctgttggag ggggcaggag tctgcactcc ctgtattcac tgagcgtcgt
     6421 ctaataaaga tgtctattga tctcttttag tgtgaatcat gtctgacgag gggccaggta
     6481 caggacctgg aaatggccta ggagagaagg gagacacatc tggaccagaa ggctccggcg
     6541 gcagtggacc tcaaagaaga gggggtgata accatggacg aggacgggga agaggacgag
     6601 gacgaggagg cggaagacca ggagccccgg gcggctcagg atcagggcca agacatagag
     6661 atggtgtccg gagaccccaa aaacgtccaa gttgcattgg ctgcaaaggg acccacggtg
     6721 gaacaggagc aggagcagga gcgggagggg caggagcagg aggggcagga gcaggaggag
     6781 gggcaggagc aggaggaggg gcaggagggg caggaggggc aggaggggca ggagcaggag
     6841 gaggggcagg agcaggagga ggggcaggag gggcaggagg ggcaggagca ggaggagggg
     6901 caggagcagg aggaggggca ggaggggcag gagcaggagg aggggcagga ggggcaggag
     6961 gggcaggagc aggaggaggg gcaggagcag gaggaggggc aggaggggca ggagcaggag
     7021 gaggggcagg aggggcagga ggggcaggag caggaggagg ggcaggagca ggaggggcag
     7081 gaggggcagg aggggcagga gcaggagggg caggagcagg aggaggggca ggaggggcag
     7141 gaggggcagg agcaggaggg gcaggagcag gaggggcagg agcaggaggg gcaggagcag
     7201 gaggggcagg aggggcagga gcaggagggg caggaggggc aggagcagga ggggcaggag
     7261 gggcaggagc aggaggaggg gcaggagggg caggagcagg aggaggggca ggaggggcag
     7321 gagcaggagg ggcaggaggg gcaggagcag gaggggcagg aggggcagga gcaggagggg
     7381 caggaggggc aggagcagga ggaggggcag gagcaggagg ggcaggagca ggaggtggag
     7441 gccggggtcg aggaggcagt ggaggccggg gtcgaggagg tagtggaggc cggggtcgag
     7501 gaggtagtgg aggccgccgg ggtagaggac gtgaaagagc caggggggga agtcgtgaaa
     7561 gagccagggg gagaggtcgt ggacgtggag aaaagaggcc caggagtccc agtagtcagt
     7621 catcatcatc cgggtctcca ccgcgcaggc cccctccagg tagaaggcca tttttccacc
     7681 ctgtagggga agccgattat tttgaatacc accaagaagg tggcccagat ggtgagcctg
     7741 acgtgccccc gggagcgata gagcagggcc ccgcagatga cccaggagaa ggcccaagca
     7801 ctggaccccg gggtcagggt gatggaggca ggcgcaaaaa aggagggtgg tttggaaagc
     7861 atcgtggtca aggaggttcc aacccgaaat ttgagaacat tgcagaaggt ttaagagctc
     7921 tcctggctag gagtcacgta gaaaggacta ccgacgaagg aacttgggtc gccggtgtgt
     7981 tcgtatatgg aggtagtaag acctcccttt acaacctaag gcgaggaact gcccttgcta
     8041 ttccacaatg tcgtcttaca ccattgagtc gtctcccctt tggaatggcc cctggacccg
     8101 gcccacaacc tggcccgcta agggagtcca ttgtctgtta tttcatggtc tttttacaaa
     8161 ctcatatatt tgctgaggtt ttgaaggatg cgattaagga ccttgttatg acaaagcccg
     8221 ctcctacctg caatatcagg gtgactgtgt gcagctttga cgatggagta gatttgcctc
     8281 cctggtttcc acctatggtg gaaggggctg ccgcggaggg tgatgacgga gatgacggag
     8341 atgaaggagg tgatggagat gagggtgagg aagggcagga gtgatgtaac ttgttaggag
     8401 acgccctcaa tcgtattaaa agccgtgtat tcccccgcac taaagaataa atccccagta
     8461 gacatcatgc gtgctgttgg tgtatttctg gccatctgtc ttgtcaccat tttcgtcctc
     8521 ccaacatggg gcaattgccg gaacccttaa tataacttcg tataatgtat gctatacgaa
     8581 gttattaggt ccctcgaaga ggttcactag cggatctcaa ttgggcatac ccatgttgtc
     8641 acgtcactca gctccgcgct caacaccttc tcgcgttgga aaacattagc gacatttacc
     8701 tggtgagcaa tcagacatgc gacggcttta gcctggcctc cttaaattca cctaagaatg
     8761 ggagcaacca gcaggaaaag gacaagcagc gaaaattcac gcccccttgg gaggtggcgg
     8821 catatgcaaa ggatagcact cccactctac tactgggtat catatgctga ctgtatatgc
     8881 atgaggatag catatgctac ccggatacag attaggatag catatactac ccagatatag
     8941 attaggatag catatgctac ccagatatag attaggatag cctatgctac ccagatataa
     9001 attaggatag catatactac ccagatatag attaggatag catatgctac ccagatatag
     9061 attaggatag cctatgctac ccagatatag attaggatag catatgctac ccagatatag
     9121 attaggatag catatgctat ccagatattt gggtagtata tgctacccag atataaatta
     9181 ggatagcata tactacccta atctctatta ggatagcata tgctacccgg atacagatta
     9241 ggatagcata tactacccag atatagatta ggatagcata tgctacccag atatagatta
     9301 ggatagccta tgctacccag atataaatta ggatagcata tactacccag atatagatta
     9361 ggatagcata tgctacccag atatagatta ggatagccta tgctacccag atatagatta
     9421 ggatagcata tgctatccag atatttgggt agtatatgct acccatggca acattagccc
     9481 accgtgctct cagcgacctc gtgaatatga ggaccaacaa ccctgtgctt ggcgctcagg
     9541 cgcaagtgtg tgtaatttgt cctccagatc gcagcaatcg cgcccctatc ttggcccgcc
     9601 cacctactta tgcaggtatt ccccggggtg ccattagtgg ttttgtgggc aagtggtttg
     9661 accgcagtgg ttagcggggt tacaatcagc caagttatta cacccttatt ttacagtcca
     9721 aaaccgcagg gcggcgtgtg ggggctgacg cgtgccccca ctccacaatt tcaaaaaaaa
     9781 gagtggccac ttgtctttgt ttatgggccc cattggcgtg gagccccgtt taattttcgg
     9841 gggtgttaga gacaaccagt ggagtccgct gctgtcggcg tccactctct ttccccttgt
     9901 tacaaataga gtgtaacaac atggttcacc tgtcttggtc cctgcctggg acacatctta
     9961 ataaccccag tatcatattg cactaggatt atgtgttgcc catagccata aattcgtgtg
    10021 agatggacat ccagtcttta cggcttgtcc ccaccccatg gatttctatt gttaaagata
    10081 ttcagaatgt ttcattccta cactagtatt tattgcccaa ggggtttgtg agggttatat
    10141 tggtgtcata gcacaatgcc accactgaac cccccgtcca aattttattc tgggggcgtc
    10201 acctgaaacc ttgttttcga gcacctcaca tacaccttac tgttcacaac tcagcagtta
    10261 ttctattagc taaacgaagg agaatgaaga agcaggcgaa gattcaggag agttcactgc
    10321 ccgctccttg atcttcagcc actgcccttg tgactaaaat ggttcactac cctcgtggaa
    10381 tcctgacccc atgtaaataa aaccgtgaca gctcatgggg tgggagatat cgctgttcct
    10441 taggaccctt ttactaaccc taattcgata gcatatgctt cccgttgggt aacatatgct
    10501 attgaattag ggttagtctg gatagtatat actactaccc gggaagcata tgctacccgt
    10561 ttagggttaa caagggggcc ttataaacac tattgctaat gccctcttga gggtccgctt
    10621 atcggtagct acacaggccc ctctgattga cgttggtgta gcctcccgta gtcttcctgg
    10681 gcccctggga ggtacatgtc ccccagcatt ggtgtaagag cttcagccaa gagttacaca
    10741 taaaggcaat gttgtgttgc agtccacaga ctgcaaagtc tgctccagga tgaaagccac
    10801 tcagtgttgg caaatgtgca catccattta taaggatgtc aactacagtc agagaacccc
    10861 tttgtgtttg gtcccccccc gtgtcacatg tggaacaggg cccagttggc aagttgtacc
    10921 aaccaactga agggattaca t
//
