LOCUS       sequence-123713-         553 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses K295A mutant of mannitol 2-dehydrogenase from Pseudomonas 
            fluorescens, codon optimized for expression in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Lucas JE, Siegel JB
  TITLE     Quantitative functional characterization of conserved molecular 
            interactions in the active site of mannitol 2-dehydrogenase.
  JOURNAL   Protein Sci. 2015 Jun;24(6):936-45. doi: 10.1002/pro.2669. Epub 2015
            Apr 2.
  PUBMED    25752240
REFERENCE   2  (bases 1 to 553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2669"; 
            journalName: "Protein Sci"; date: "2015-06"; volume: "24"; issue: 
            "6"; pages: "936-45"
COMMENT     Sequence Label: pfMDH_K295A
FEATURES             Location/Qualifiers
     source          1..553
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..30)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agccggatct cagtggtggt ggtggtggtg ctcgaggcta ccaacaggtt ttttcagcag
       61 atgtttcagg gtggtggtaa caccattatc acgcaggcta ccataacaac gttcaaatgc
      121 ggcaacaaat tccggactat tcggaattgc ggtgccaaaa atttcttcaa ctgccagcag
      181 acgctggcta atcagtgcat catctgaaac cagaccctga caaaattctg cacgcggatc
      241 aggaatggta tagctcacac cattttcatc aacgcctttc agatacagtg cccatgctgc
      301 aacaaccagt gctgcacgtt cggtttcacg accatctgca atcagacgat taattgtcgg
      361 aacggtaaat ttaggaaatt tgctgctacc atcactacaa acacgttcca gctgatctgc
      421 aattgcctga ttgctaaaac gatcaaccag ggtctgttta taatcggtca gatcaatacc
      481 cggaaccgga gccagattcg gggtcacatc cagatccata tatgcacgca tataggcaac
      541 aaacagcgga tcg
//
