LOCUS       sequence-123715-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Q157TAG GFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, 
            Isaacs FJ, Rinehart J
  TITLE     A flexible codon in genomically recoded Escherichia coli permits 
            programmable protein phosphorylation.
  JOURNAL   Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
  PUBMED    26350500
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-09-9"; volume: "6:8130. doi"; pages: " 
            10.1038/ncomms9130"
COMMENT     Sequence Label: GFP Q157TAG
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..56)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(63..776)
                     /codon_start=1
                     /product="GFP variant optimized for excitation by UV light"
                     /label=GFPuv
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADK*KNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(103..123)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     718..746
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     RBS             783..794
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 agcagccgga tcaagctttg aattgagctc gcccttcagt ggtggtggtg gtggtgctcg
       61 agtttgtaga gctcatccat gccatgtgta atcccagcag cagttacaaa ctcaagaagg
      121 accatgtggt cacgcttttc gttgggatct ttcgaaaggg cagattgtgt cgacaggtaa
      181 tggttgtctg gtaaaaggac agggccatcg ccaattggag tattttgttg ataatggtct
      241 gctagttgaa cggatccatc ttcaatgttg tggcgaattt tgaagttagc tttgattcca
      301 ttcttctatt tgtctgccgt gatgtataca ttgtgtgagt tatagttgta ttcgagtttg
      361 tgtccgagaa tgtttccatc ttctttaaaa tcaatacctt ttaactcgat acgattaaca
      421 agggtatcac cttcaaactt gacttcagca cgcgtcttgt agttcccgtc atctttgaaa
      481 gatatagtgc gttcctgtac ataaccttcg ggcatggcac tcttgaaaaa gtcatgccgt
      541 ttcatgtgat ccggataacg ggaaaagcat tgaacaccat aagagaaagt agtgacaagt
      601 gttggccatg gaacaggtag ttttccagta gtgcaaataa atttaagggt aagttttccg
      661 tatgttgcat caccttcacc ctctccactg acagaaaatt tgtgcccatt aacatcacca
      721 tctaattcaa caagaattgg gacaactcca gtgaaaagtt cttctccttt actcatggta
      781 cctttctcct ctttaatgaa ttcggtcag
//
