LOCUS       sequence-123717-        1109 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N191A mutant of mannitol 2-dehydrogenase from Pseudomonas 
            fluorescens, codon optimized for expression in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Lucas JE, Siegel JB
  TITLE     Quantitative functional characterization of conserved molecular 
            interactions in the active site of mannitol 2-dehydrogenase.
  JOURNAL   Protein Sci. 2015 Jun;24(6):936-45. doi: 10.1002/pro.2669. Epub 2015
            Apr 2.
  PUBMED    25752240
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2669"; 
            journalName: "Protein Sci"; date: "2015-06"; volume: "24"; issue: 
            "6"; pages: "936-45"
COMMENT     Sequence Label: pfMDH_N191A
FEATURES             Location/Qualifiers
     source          1..1109
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     72..91
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        72..90
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    91..115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             130..152
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tcgatctcga
       61 tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct
      121 agaaataatt ttgtttaact ttaagaagga gatatacata tgaaactgaa caaacagaat
      181 ctgacccagc tggcaccgga agttaaactg cctgcatata ccctggcaga tacccgtcag
      241 ggtattgcac atattggtgt tggtggtttt catcgtgcac atcaggcata ttataccgat
      301 gcactgatga atacaggtga aggtctggat tggagcattt gcggtgttgg tctgcgtagc
      361 gaagatcgta aagcacgtga tgatctggca ggtcaggatt acctgtttac cctgtatgaa
      421 ctgggcgata ccgatgatac cgaagttcgt gttattggta gcattagcga tatgctgctg
      481 gcagaagata gcgcacaggc actgattgat aaactggcaa gtccggaaat tcgtattgtt
      541 agcctgacca ttaccgaagg tggttattgt attgatgata gcaacggtga atttatggca
      601 catctgccgc agattcagca cgatctggca catccgagca gcccgaaaac cgtttttggt
      661 tttatttgtg cagcactgac acagcgtcgt gcagcaggta ttccggcatt taccgttatg
      721 agctgtgatg cgctgccgca taatggtgca gttacccgta aagccctgct ggcatttgca
      781 gccctgcata atgcagaact gcatgattgg attaaagccc atgttagctt tccgaatgca
      841 atggttgatc gtattacccc gatgaccagc accgcacatc gtctgcaact gcatgacgaa
      901 catggtattg atgatgcatg gcctgttgtt tgtgaaccgt ttgttcagtg ggttctggaa
      961 gataaatttg ttaatggtcg tccggcatgg gaaaaagttg gtgttcagtt taccgatgat
     1021 gttaccccgt atgaagaaat gaaaattggt ctgctgaatg gtagccatct ggcactgacc
     1081 tatctgggtt ttctgaaagg ttatcgttt
//
