LOCUS       sequence-123720-         625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N191A mutant of mannitol 2-dehydrogenase from Pseudomonas 
            fluorescens, codon optimized for expression in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Lucas JE, Siegel JB
  TITLE     Quantitative functional characterization of conserved molecular 
            interactions in the active site of mannitol 2-dehydrogenase.
  JOURNAL   Protein Sci. 2015 Jun;24(6):936-45. doi: 10.1002/pro.2669. Epub 2015
            Apr 2.
  PUBMED    25752240
REFERENCE   2  (bases 1 to 625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2669"; 
            journalName: "Protein Sci"; date: "2015-06"; volume: "24"; issue: 
            "6"; pages: "936-45"
COMMENT     Sequence Label: pfMDH_N191A
FEATURES             Location/Qualifiers
     source          1..625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..29)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gccggatctc agtggtggtg gtggtggtgc tcgaggctac caacaggttt tttcagcaga
       61 tgtttcaggg tggtggtaac accattatca cgcaggctac cataacaacg ttcaaatgcg
      121 gcaacaaatt ccggactatt cggaattgcg gtgccaaaaa tttcttcaac tgccagcaga
      181 cgctggctaa tcagtgcatc atctgaaacc agaccctgac aaaattctgc acgcggatca
      241 ggaatggtat agctcacacc attttcatca acgcctttca gatacagtgc ccatgctgca
      301 acaaccagtg ctgcacgttc ggtttcacga ccatctgcaa tcagacgatt aattgtcgga
      361 acggtaaatt taggaaattt gctgctacca tcactacaaa cacgttccag ctgatctgca
      421 attgcctgat tgctaaaacg atcaaccagg gtctgtttat aatcggtcag atcaataccc
      481 ggaaccggag ccagattcgg ggtcacatcc agatccatat atgcacgcat ataggcaaca
      541 aacagcggat cgttcatggt ttcatgcaca aaacgataac ctttcagaaa acccagatag
      601 gtcagtgcca gatggctacc attca
//
