LOCUS       sequence-123723-        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a recombineering cassette for tagging a target gene within a
            genomic clone with a fluorescent protein. Cassette: bicistronic; 
            FRT-galK-FRT in 2nd GFP intron;use C-term after STOP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Tursun B, Cochella L, Carrera I, Hobert O
  TITLE     A toolkit and robust pipeline for the generation of fosmid-based 
            reporter genes in C. elegans.
  JOURNAL   PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 
            2009 Mar 4.
  PUBMED    19259264
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009"; volume: "4(3):e4625. doi"; pages: " 
            10.1371/journal.pone.0004625. Epub 2009 Mar 4"
COMMENT     Sequence Label: pBALU9
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             326..346
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(389..417)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    complement(757..790)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        794..841
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 ggcgaattgg gccctctaga tgcatgctcg agcggccgcc agtgtgatgg atatctgcag
       61 acctctccct tgctgtctca tcctactttc acctagttaa ctgcttgtct taaaatctat
      121 gcttctcttt agtatctaaa attttcctag aagcttacaa gtatataaat ggtctcttct
      181 caataaaggt tgtatattta ttcatcttat tgaatctgcc atttcctcgt ttttgcgagt
      241 ttatatacct tccaattttc tttctattgt attttcaact tctaatttta attcagggaa
      301 actgcttcaa cgcatcatga ccgctccaaa gaagaaacgc aaagtaccgg tagaaaaaat
      361 gagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
      421 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
      481 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggg taagtttaaa
      541 catatatata ctaactaacc ctgattattt aaattttcag ccaacacttg tcactacttt
      601 ctgttatggt gttcaatgct tctcgagata cccagatcat atgaaacggc atgacttttt
      661 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
      721 gaactacaag acacgtaagt ttaaacagtt cggtacgaag ttcctatact ttctagagaa
      781 taggaacttc cctgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
      841 caaggtgagg aactaaaccc aggaggcaga tcatgagtct gaaagaaaaa acacaatctc
      901 tgtttgccaa cgcatttggc taccctgcca ctcacaccat tcaggcgcct ggccgcgtga
      961 atttgattgg tgaacacacc gactacaacg acggtttcgt tctgccctgc gcgattgatt
     1021 atcaaaccgt gatcagttgt gcaccacgcg atgaccgtaa agttcg
//
