LOCUS       sequence-123724-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal MBP tagged human MEK1 gene S218TAG for bacterial 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, 
            Isaacs FJ, Rinehart J
  TITLE     A flexible codon in genomically recoded Escherichia coli permits 
            programmable protein phosphorylation.
  JOURNAL   Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
  PUBMED    26350500
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-09-9"; volume: "6:8130. doi"; pages: " 
            10.1038/ncomms9130"
COMMENT     Sequence Label: MBP-MEK1 S218TAG
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..40
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             41..61
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 caacaataac aataacaaca acctcgggat cgagggaagg gaaaatctgt attttcaagg
       61 tcatatgccg aagaagaaac cgaccccgat ccagctgaac ccggctccgg acggttctgc
      121 tgttaacggc acctcttctg ctgaaaccaa cctggaagct ctgcaaaaga aactggaaga
      181 actggaactg gacgaacagc agcgtaaacg tctggaagcg ttcctgaccc agaaacagaa
      241 agttggtgaa ctgaaagacg acgacttcga aaaaatctct gaactgggtg ctggtaacgg
      301 tggtgttgtt ttcaaagttt ctcacaaacc gtccggtctg gttatggctc gtaaactgat
      361 ccacctggaa atcaaaccgg ctatccgtaa ccagatcatc cgtgaactgc aagttctgca
      421 cgaatgcaac tctccgtaca tcgttggttt ctacggtgct ttctactctg acggtgaaat
      481 ctctatctgc atggaacaca tggacggtgg ttctctggac caggttctga aaaaagctgg
      541 tcgtatcccg gaacagatcc tgggtaaagt ttctatcgct gttatcaaag gtctgaccta
      601 cctgcgtgaa aaacacaaaa tcatgcaccg tgacgttaaa ccgtctaaca tcctggttaa
      661 ctctcgtggt gaaatcaaac tgtgcgactt cggtgtttct ggtcagctga tcgactagat
      721 ggctaactct ttcgttggca cccgttctta catgtctccg gaacgtctgc aaggcaccca
      781 ctactctgtt cagtctgaca tctggtctat gggtctgtct ctggttgaaa tggctgttgg
      841 tcgttacccg atcccgccgc cggacgctaa agaactggaa ctgatgttc
//
