LOCUS       sequence-123725-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a recombineering cassette for tagging a target gene within a
            genomic clone with a fluorescent protein. Cassette: bicistronic; 
            FRT-galK-FRT in 2nd GFP intron;use C-term after STOP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Tursun B, Cochella L, Carrera I, Hobert O
  TITLE     A toolkit and robust pipeline for the generation of fosmid-based 
            reporter genes in C. elegans.
  JOURNAL   PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 
            2009 Mar 4.
  PUBMED    19259264
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009"; volume: "4(3):e4625. doi"; pages: " 
            10.1371/journal.pone.0004625. Epub 2009 Mar 4"
COMMENT     Sequence Label: pBALU9
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(88..108)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    517..550
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     559..575
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     559..575
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gagctcggat cactagtaac ggccgccagt gtgctggaat tcgccctttt gtatagttca
       61 tccatgccat gtgtaatccc agcagctgtt acaaactcaa gaaggaccat gtggtctctc
      121 ttttcgttgg gatctttcga aagggcagat tgtgtggaca ggtaatggtt gtctggtaaa
      181 aggacagggc catcgccaat tggagtattt tgttgataat ggtctgctag ttgaacgctt
      241 ccatcttcaa tgttgtgtct aattttgaag ttctgaaaat ttaaatcaga ttagttagtt
      301 agtaaaatca tgtttaaact tacaactttg attccattct tttgtttgtc tgccatgatg
      361 tatacattgt gtgagttata gttgtattcc aatttgtgtc caagaatgtt tccatcttct
      421 ttaaaatcaa taccttttaa ctcgattcta ttaacaaggg tatcaccttc aaacttgact
      481 tcagcacctg aaaatttaaa tatgtatggt tagttagaag ttcctattct ctagaaagta
      541 taggaacttc gcggccgctc tagaactagt ggatcctcag cactgtcctg ctccttgtga
      601 tggtttacaa acgtaaaaag tctctttaat acctgttttt gcttcatatt gttcagcaac
      661 agcttgctgt acggcaggca ccagctcttc cgggatcagc gcgacgatac agccgccaaa
      721 tccgccgccg gtcatgcgta cgccaccttt gtcgccaatc acagctttga cgatttctac
      781 cagagtgtca atttgcggca cggtgatttc gaaatcatcg cgcatagagg catgagactc
      841 cgccatcaac tcgcccatac gtttcaggtc gccttgctcc agcgcgctgg cagcttcaac
      901 ggtgcgggcg ttttcagtca gtatatgacg cacgcgtttt gccacgatcg ggtccagttc
      961 atgc
//
