LOCUS       sequence-123726-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal MBP tagged human MEK1 gene S218TAG for bacterial 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, 
            Isaacs FJ, Rinehart J
  TITLE     A flexible codon in genomically recoded Escherichia coli permits 
            programmable protein phosphorylation.
  JOURNAL   Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
  PUBMED    26350500
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-09-9"; volume: "6:8130. doi"; pages: " 
            10.1038/ncomms9130"
COMMENT     Sequence Label: MBP-MEK1 S218TAG
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(58..75)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcagccggat caagctagct tgtcgacgga gctcgaattc ggatccggcg cgccttagtg
       61 atggtggtga tgatgcgcag ctgcggctgc cacaccagca gcgtgggtcg gggtagacgg
      121 ctggttcaga ccgatggtag agcacagcca accagcgaag tcaacttctt cagcgtcaga
      181 acgtttgatg aaagcgtgaa ccatcagctg tttcaggtca gcacgttcag ccgggttttt
      241 gatcaggcat ttgttaacga agtcctggaa ctccagagag aaaacaccag acggcagttt
      301 cggaggcggt tcgttaacga tgtagtccag cagttcgaag atagccatcg gcggacgaga
      361 gtccataccg taagaagaca gcggacgacc cggagtacgc ggacgcggcg gggtttcagc
      421 agcgtcacct tcaacctggc aaccgaacat cagttccagt tctttagcgt ccggcggcgg
      481 gatcgggtaa cgaccaacag ccatttcaac cagagacaga cccatagacc agatgtcaga
      541 ctgaacagag tagtgggtgc cttgcagacg ttccggagac atgtaagaac gggtgccaac
      601 gaaagagtta gccatctagt cgatcagctg accagaaaca ccgaagtcgc acagtttgat
      661 ttcaccacga gagttaacca ggatgttaga cggtttaacg tcacggtgca tgattttgtg
      721 tttttcacgc aggtaggtca gacctttgat aacagcgata gaaactttac cca
//
