LOCUS       sequence-123729-         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal MBP tagged human MEK1 gene S222TAG for bacterial 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, 
            Isaacs FJ, Rinehart J
  TITLE     A flexible codon in genomically recoded Escherichia coli permits 
            programmable protein phosphorylation.
  JOURNAL   Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
  PUBMED    26350500
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-09-9"; volume: "6:8130. doi"; pages: " 
            10.1038/ncomms9130"
COMMENT     Sequence Label: MBP-MEK1 S222TAG
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(57..74)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cagccggatc aagctagctt gtcgacggag ctcgaattcg gatccggcgc gccttagtga
       61 tggtggtgat gatgcgcagc tgcggctgcc acaccagcag cgtgggtcgg ggtagacggc
      121 tggttcagac cgatggtaga gcacagccaa ccagcgaagt caacttcttc agcgtcagaa
      181 cgtttgatga aagcgtgaac catcagctgt ttcaggtcag cacgttcagc cgggtttttg
      241 atcaggcatt tgttaacgaa gtcctggaac tccagagaga aaacaccaga cggcagtttc
      301 ggaggcggtt cgttaacgat gtagtccagc agttcgaaga tagccatcgg cggacgagag
      361 tccataccgt aagaagacag cggacgaccc ggagtacgcg gacgcggcgg ggtttcagca
      421 gcgtcacctt caacctggca accgaacatc agttccagtt ctttagcgtc cggcggcggg
      481 atcgggtaac gaccaacagc catttcaacc agagacagac ccatagacca gatgtcagac
      541 tgaacagagt agtgggtgcc ttgcagacgt tccggagaca tgtaagaacg ggtgccaacg
      601 aactagttag ccatagagtc gatcagctga ccagaaacac cgaagtcgca cagtttgatt
      661 tcaccacgag agttaaccag gatgttagac ggtttaacgt cacggtgcat gattttgtgt
      721 ttttcacgca ggtaggtcag acctttgata acagcgatag aaactttacc caggatctgt
      781 tccgggatac gaccagcttt tttcagaacc tggtccagag aaccaccgtc catgtgttcc
      841 atgcagatag agatttcacc gtcagagtag aaagcacc
//
