LOCUS       sequence-123730-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Beta lactamase S68TAG gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, 
            Isaacs FJ, Rinehart J
  TITLE     A flexible codon in genomically recoded Escherichia coli permits 
            programmable protein phosphorylation.
  JOURNAL   Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
  PUBMED    26350500
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-09-9"; volume: "6:8130. doi"; pages: " 
            10.1038/ncomms9130"
COMMENT     Sequence Label: Beta lactamase S68TAG
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..414)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     361..380
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(599..703)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
       61 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
      121 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
      181 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
      241 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
      301 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
      361 ataataccgc gccacatagc agaactttaa aagtctacat cattggaaaa cgttcttcgg
      421 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
      481 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
      541 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
      601 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
      661 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
      721 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
      781 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat ca
//
