LOCUS       sequence-123749-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S2TAG GFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, 
            Isaacs FJ, Rinehart J
  TITLE     A flexible codon in genomically recoded Escherichia coli permits 
            programmable protein phosphorylation.
  JOURNAL   Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
  PUBMED    26350500
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-09-9"; volume: "6:8130. doi"; pages: " 
            10.1038/ncomms9130"
COMMENT     Sequence Label: GFP S2TAG
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(37..54)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(61..774)
                     /codon_start=1
                     /product="GFP variant optimized for excitation by UV light"
                     /label=GFPuv
                     /translation="M*KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(101..121)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     716..744
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     RBS             781..792
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 cagccggatc aagctttgaa ttgagctcgc ccttcagtgg tggtggtggt ggtgctcgag
       61 tttgtagagc tcatccatgc catgtgtaat cccagcagca gttacaaact caagaaggac
      121 catgtggtca cgcttttcgt tgggatcttt cgaaagggca gattgtgtcg acaggtaatg
      181 gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc
      241 tagttgaacg gatccatctt caatgttgtg gcgaattttg aagttagctt tgattccatt
      301 cttttgtttg tctgccgtga tgtatacatt gtgtgagtta tagttgtatt cgagtttgtg
      361 tccgagaatg tttccatctt ctttaaaatc aatacctttt aactcgatac gattaacaag
      421 ggtatcacct tcaaacttga cttcagcacg cgtcttgtag ttcccgtcat ctttgaaaga
      481 tatagtgcgt tcctgtacat aaccttcggg catggcactc ttgaaaaagt catgccgttt
      541 catgtgatcc ggataacggg aaaagcattg aacaccataa gagaaagtag tgacaagtgt
      601 tggccatgga acaggtagtt ttccagtagt gcaaataaat ttaagggtaa gttttccgta
      661 tgttgcatca ccttcaccct ctccactgac agaaaatttg tgcccattaa catcaccatc
      721 taattcaaca agaattggga caactccagt gaaaagttct tctcctttct acatggtacc
      781 tttctcctct ttaa
//
