LOCUS       sequence-123765-        6249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses M2-SH3PXD2A in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6249)
  AUTHORS   Schaffer BE, Levin RS, Hertz NT, Maures TJ, Schoof ML, Hollstein PE,
            Benayoun BA, Banko MR, Shaw RJ, Shokat KM, Brunet A
  TITLE     Identification of AMPK Phosphorylation Sites Reveals a Network of 
            Proteins Involved in Cell Invasion and Facilitates Large-Scale 
            Substrate Prediction.
  JOURNAL   Cell Metab. 2015 Nov 3;22(5):907-21. doi: 
            10.1016/j.cmet.2015.09.009. Epub 2015 Oct 8.
  PUBMED    26456332
REFERENCE   2  (bases 1 to 6249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.09.009"; journalName: "Cell Metab"; date: 
            "2015-11-3- 3"; volume: "22"; issue: "5"; pages: "907-21"
COMMENT     Sequence Label: pECE M2-SH3PXD2A 8 serines mutated to alanines
FEATURES             Location/Qualifiers
     source          1..6249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    115..250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(153..172)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     207..226
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(309..328)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(587..604)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(758..1346)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(838..857)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1518..2309,2310..2378))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2141..2160
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2379..2483)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        2513..2704
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2536..2556)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2554..2851
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2893..2916
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 taagtaatga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
       61 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
      121 tttattgcag gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
      181 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
      241 tgtctggatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
      301 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
      361 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
      421 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
      481 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtgcggtgt gaaataccgc
      541 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
      601 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
      661 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
      721 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      781 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      841 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      901 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
      961 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1021 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1081 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1141 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     1201 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     1261 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     1321 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     1381 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     1441 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaaag tatatatgag
     1501 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     1561 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     1621 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     1681 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     1741 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     1801 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
     1861 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     1921 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     1981 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2041 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2101 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
     2161 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     2221 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     2281 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     2341 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     2401 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2461 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgc ttctgaggcg
     2521 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     2581 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     2641 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     2701 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     2761 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     2821 tattccagaa gtagtgaaga ggcttttttg gaggagatct aagcttggta ccgagctcgg
     2881 atcctcagga tggactacaa ggacgatgac gataaacccg ggaattggct cgcctactgc
     2941 gtgcaggatg ccaccgtggt ggacgtggag aagcggagga acccctccaa gcactacgta
     3001 tacataatca atgtgacctg gtctgactcc acctcccaga ctatctaccg gaggtacagc
     3061 aagttctttg acctgcagat gcagcttttg gataagtttc ccattgaagg tggccagaag
     3121 gaccccaagc aaaggatcat ccccttcctc ccaggcaaga tcctcttccg cagaagccac
     3181 atccgggacg tagctgtgaa gagactgaag cccatcgatg aatactgccg ggcacttgtc
     3241 cggctgcccc cccacatctc acagtgtgac gaagtcttcc ggttcttcga ggctcgaccc
     3301 gaggatgtca accctccaaa agaggactat ggcagttcca agaggaaatc agtgtggctg
     3361 tccagctggg ctgagtcgcc caagaaggac gtgacaggtg ccgacgccac cgccgagccc
     3421 atgatcctgg aacagtacgt ggtggtgtcc aactataaga agcaggagaa ctcggagctg
     3481 agcctccagg ccggggaggt ggtggatgtc atcgagaaga acgagagcgg ctggtggttc
     3541 gtgagcactt ctgaggagca gggctgggtc cctgccacct acctggaggc ccagaatggt
     3601 actcgggatg actccgacat caacacctct aagactggag aagaggagaa gtatgtcacc
     3661 gtgcagcctt acaccagcca aagcaaggac gagattggct ttgagaaggg cgtcacagtg
     3721 gaggtgatcc ggaagaatct ggaaggctgg tggtatatca gatacctggg caaagagggc
     3781 tgggcgccag catcctacct gaagaaggcc aaggatgacc tgccaacccg gaagaagaac
     3841 ctggccggcc cagtggagat cattgggaac atcatggaga tcagcaacct gctgaacaag
     3901 aaggcgtctg gggacaagga aactccacca gccgaaggcg agggccatga ggcccccatt
     3961 gccaagaagg agatcagcct gcccatcctc tgcaatgcct ccaatggcag tgccgtgggc
     4021 gttcctgaca ggactgtctc caggctggcc cagggctctc cagctgtggc caggattgcc
     4081 cctcagcggg cccagatcag ctccccgaac ctacggacaa gacctccacc acgcagagaa
     4141 tccagcctgg ggttccaact gccaaagcca ccagagcccc cttctgttga ggtggagtac
     4201 tacaccattg ccgaattcca gtcgtgcatt tccgatggca tcagctttcg gggtggacag
     4261 aaggcagagg tcattgataa gaactcaggt ggctggtggt acgtgcagat cggtgagaag
     4321 gagggctggg cccccgcatc atacatcgat aagcgcaaga agcccaacct gagccgccgc
     4381 acaagcacgc tgacccggcc caaggtgccc ccgccagcac cccccagcaa gcccaaggag
     4441 gccgaggagg gccctacggg ggccagtgag agccaggact ccccgcggaa gctcaagtat
     4501 gaggagcctg agtatgacat ccctgcattc ggctttgact cagagcctga gctgagcgag
     4561 gagcccgtgg aggacagagc ctcaggggag aggcggcctg cccagcccca ccggccctcg
     4621 ccggcctctt ctctgcagcg ggcccgcttc aaggtgggtg agtcttcaga ggatgtggcc
     4681 ctggaagagg agaccatcta tgagaatgag ggcttccggc catatgcaga ggacaccctg
     4741 tcagccagag gctcctccgg ggacagcgac tccccaggca gctcctcgct gtccctgacc
     4801 aggaaaaact cccccaaatc aggctccccc aagtcatcat cactcctaaa gctcaaggca
     4861 gagaagaatg cccaggcaga aatggggaag aaccactcct cagcctcctt ttcctcatcc
     4921 atcaccatca acaccacttg ctgctcctcc tcttcctcct cctcctcttc cttgtccaaa
     4981 accagtggcg acctgaagcc ccgctctgct tcggacgcag gcatccgcgg cactcccaag
     5041 gtcagggcaa agaaggatgc tgatgcgaac gctgggctga cctcctgtcc ccgggccaag
     5101 ccatcggtcc ggcccaagcc attcctaaac cgagcagagt cgcagagcca agagaagatg
     5161 gacatcagca ctttacggcg ccagctgaga cccacaggcc agctccgtgg agggctcaag
     5221 ggctccaaga gtgaggattc ggagctgccc ccgcagacgg cctccgaggc tcccagtgag
     5281 gggtctagga gaagctcatc cgacctcatc accctcccag ccaccactcc cccatgtccc
     5341 accaagaagg aatgggaagg gccagccacc tcgtacatga catgcagcgc ctaccagaag
     5401 gtccaggact cggagatcag cttccccgcg ggcgtggagg tgcaggtgct ggagaagcag
     5461 gagagcgggt ggtggtatgt gaggtttggg gagctggagg gctgggcccc ttcccactat
     5521 ttggtgctgg atgagaacga gcaacctgac ccctctggca aagagctgga cacagtgccc
     5581 gccaagggca ggcagaacga aggcaaatca gacagcctgg agaagatcga gaggcgcgtc
     5641 caagcactga acaccgtcaa ccagagcaag aaggccacgc cccccatccc ctccaaacct
     5701 cccgggggct tcggcaagac ctcaggcact ccagcggtga agatgaggaa cggagtgcgg
     5761 caggtggcgg tcaggcccca gtcggtgttt gtgtccccgc cacccaagga caacaacctg
     5821 tcctgcgccc tgcggaggaa tgagtcactc acggccactg atggcctccg aggcgtccga
     5881 cggaactcct cctttagcac tgctcgctcc gctgccgccg aggccaaggg ccgcctggcc
     5941 gaacgggctg ccagccaggg ttcagactca cccctactgc ccgcccagcg caacagcata
     6001 cccgtgtccc ctgtgcgccc caagcccatc gagaagtctc agttcatcca caataacctc
     6061 aaagatgtgt acgtctctat cgcagactac gagggggatg aggagacagc aggcttccag
     6121 gagggggtgt ccatggaggt tctggagagg aaccctaatg gctggtggta ctgccagatc
     6181 ctggatggtg tgaagccctt caaaggctgg gtgccttcca actaccttga gaaaaagaac
     6241 taatctaga
//
