LOCUS       sequence-123776-        5673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  luciferase reporter for Calb2 promoter (-838 to +80, WT).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Kresoja-Rakic J, Kapaklikaya E, Ziltener G, Dalcher D, Santoro R, 
            Christensen BC, Johnson KC, Schwaller B, Weder W, Stahel RA, 
            Felley-Bosco E
  TITLE     Identification of cis- and trans-acting elements regulating 
            calretinin expression in mesothelioma cells.
  JOURNAL   Oncotarget. 2016 Feb 1. doi: 10.18632/oncotarget.7114.
  PUBMED    26848772
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncotarget.
            2016 Feb 1. doi: 10.18632/oncotarget.7114."
COMMENT     Sequence Label: pGL3-B-838;+80pCalb2
FEATURES             Location/Qualifiers
     source          1..5673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             943..2595
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1009..1027)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2636..2757
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2647..2666)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2701..2720
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3005..3022)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3176..3764)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3256..3275)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3935..4726,4727..4795))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4558..4577
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4796..4900)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4927..5382
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5014..5033)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5224..5245
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5513..5561
                     /note="synthetic polyadenylation signal"
     misc_feature    5575..5666
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5615..5634
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcacat tcccacgatg tccctaggtg ggcgtggtta
       61 agacctggta cgggttgatc tagttctgcc accccctggc caaatgtcta aaagcccccc
      121 agagcatggc cagcgtgagg caggtaccag gggtggaggg aggctccgag gggacggata
      181 tacgaagacc caaacagaca gtggaagccc cccaccccca ccccacacca cttccatcgg
      241 aatcctcccg gggcactgct gattccagct gctccccact aaagccttga gaactcttgg
      301 ctgctctgaa agactgagcc ccatgaagga gccacgtgcg gcgtggaaag agtgctgagt
      361 tcaaattgta gccctgccac taatttgctg ggccagtcac ttaatcatct gaagtcacaa
      421 gtacctcatc agaaaagtgg tcccagctct tcctgctgtg gaaggatcag aagagaggag
      481 gcacgacaga gacctagtga actccgaagc ccgagtgcta aatatttgtc aagtttgtgt
      541 tagtattact attagtgttg ttactgctgt tattattatt gctactgcca gcaataataa
      601 gtggtggatg tactcaagac ggtcgggagg gaaggcaagg gcagcctctc cctcattttc
      661 accgaaaatc ctccgggtgt ccctggcccc gcgccgaggg gtctcagcgc agaggtaagg
      721 gccctctagg agtccgggcc gagcctctcg cgccgccgcc cccgccgcgc cgcgccccgg
      781 tcggattccc tgagcgcgcg cgcccccttc tggcggccgg gcgcaggcgc aggctccaga
      841 gcgtatataa gggcagcgtg gcgcacaacc ccagcgcgag tgccagagcc cagccggcgc
      901 ggagcgggag cggtgcaggc tgaggtctcc gagcggctcg ccatggaaga cgccaaaaac
      961 ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg agagcaactg
     1021 cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat
     1081 atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct
     1141 atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt
     1201 caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac
     1261 gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc taccgtggtg
     1321 ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc
     1381 caaaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg
     1441 ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtgcc agagtccttc
     1501 gatagggaca agacaattgc actgatcatg aactcctctg gatctactgg tctgcctaaa
     1561 ggtgtcgctc tgcctcatag aactgcctgc gtgagattct cgcatgccag agatcctatt
     1621 tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt
     1681 tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat
     1741 agatttgaag aagagctgtt tctgaggagc cttcaggatt acaagattca aagtgcgctg
     1801 ctggtgccaa ccctattctc cttcttcgcc aaaagcactc tgattgacaa atacgattta
     1861 tctaatttac acgaaattgc ttctggtggc gctcccctct ctaaggaagt cggggaagcg
     1921 gttgccaaga ggttccatct gccaggtatc aggcaaggat atgggctcac tgagactaca
     1981 tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt
     2041 ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcaa
     2101 agaggcgaac tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa caatccggaa
     2161 gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg
     2221 gacgaagacg aacacttctt catcgttgac cgcctgaagt ctctgattaa gtacaaaggc
     2281 tatcaggtgg ctcccgctga attggaatcc atcttgctcc aacaccccaa catcttcgac
     2341 gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt
     2401 ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta
     2461 acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt
     2521 accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga
     2581 aagatcgccg tgtaattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga
     2641 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     2701 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     2761 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     2821 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtcgac cgatgccctt
     2881 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
     2941 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg
     3001 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     3061 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     3121 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3181 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3241 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3301 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3361 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3421 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3481 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3541 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3601 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     3661 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     3721 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     3781 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     3841 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     3901 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     3961 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     4021 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     4081 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     4141 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     4201 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     4261 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     4321 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     4381 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     4441 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     4501 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     4561 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     4621 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     4681 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     4741 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     4801 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     4861 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     4921 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4981 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     5041 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     5101 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     5161 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     5221 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     5281 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     5341 aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcccattcg ccattcaggc
     5401 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagcccaagc
     5461 taccatgata agtaagtaat attaaggtac gggaggtact tggagcggcc gcaataaaat
     5521 atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag tactaacata
     5581 cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg tccccagtgc
     5641 aagtgcaggt gccagaacat ttctctatcg ata
//
