LOCUS       sequence-123785-         178 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 nuclease under control of pTet promoter with ssrA tag and 
            constitutive tetR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 178)
  AUTHORS   Reisch CR, Prather KL
  TITLE     The no-SCAR (Scarless Cas9 Assisted Recombineering) system for 
            genome editing in Escherichia coli.
  JOURNAL   Sci Rep. 2015 Oct 14;5:15096. doi: 10.1038/srep15096.
  PUBMED    26463009
REFERENCE   2  (bases 1 to 178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-10-14"; volume: "5:15096. doi"; pages: " 
            10.1038/srep15096"
COMMENT     Sequence Label: pCas9-CR4
FEATURES             Location/Qualifiers
     source          1..178
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccagatatcg
       61 acgtcttaag acccactttc acatttaagt tgtttttcta atccgcatat gatcaattca
      121 aggccgaata agaaggctgg ctctgcacct tggtgatcaa ataattcgat agcttgtc
//
