LOCUS       sequence-123839-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4A) mCherry 
            Non functional sensor with non-phosphorylatable NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Durandau E, Aymoz D, Pelet S
  TITLE     Dynamic single cell measurements of kinase activity by synthetic 
            kinase activity relocation sensors.
  JOURNAL   BMC Biol. 2015 Aug 1;13:55. doi: 10.1186/s12915-015-0163-z.
  PUBMED    26231587
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2015-08-1"; volume: "13:55. doi"; pages: " 
            10.1186/s12915-015-0163-z"
COMMENT     Sequence Label: pED53 pRS306 pRPS2-Ste7DS NLS(4A)-mCherry
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aattcccact tttactatgc ttcaattgac ccttcggtat ctcagcatct aggtagcttt
       61 cattgagatt catagttccg atgtactgat tctacgtttg tgtactttga tagacttttt
      121 tattcaattc gtattcacta ctatctttgt ctcgtaccgc ggtttttatc ctttatttca
      181 gccctacgag atagaacgat ggagcctagt aataccattt tatgtaagca aaataagaag
      241 aaagactaaa agaattagga tatgttgtta atcagggata aaggtacgca tactagcctt
      301 actacgtaaa tataaggtta cggtttacga tcactattgc aagatactat tgtttagcat
      361 tggcaaagac ttgacgggaa atctttgatt gggcccaacc atattcatta aagatccggg
      421 atgtgagctt gaactgcttt tcgacgcgaa gaaggtttac acgtatttta gtaattggct
      481 tattcactaa ggattcttaa ggttttctta atagttttct acgtcggcat gcgattgttt
      541 ggtttagaag actgctttct aaatatggtt gggtgtattt aagctagacc catacacccg
      601 ctctatggga ttatttactt gtttgaattt taagatttgt gataatggaa ctggacgcaa
      661 acatttgatg gaaaacgcat gtcatcatta acgaggtaac gtaggtatct gtcctgcctt
      721 agtattgcac gcagcttccc aggacgccta gctatttttt catctattcc
//
