LOCUS       sequence-123870-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses H303A mutant of mannitol 2-dehydrogenase from Pseudomonas 
            fluorescens, codon optimized for expression in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Lucas JE, Siegel JB
  TITLE     Quantitative functional characterization of conserved molecular 
            interactions in the active site of mannitol 2-dehydrogenase.
  JOURNAL   Protein Sci. 2015 Jun;24(6):936-45. doi: 10.1002/pro.2669. Epub 2015
            Apr 2.
  PUBMED    25752240
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2669"; 
            journalName: "Protein Sci"; date: "2015-06"; volume: "24"; issue: 
            "6"; pages: "936-45"
COMMENT     Sequence Label: pfMDH_H303A
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gaggctacca
       61 acaggttttt tcagcagatg tttcagggtg gtggtaacac cattatcacg caggctacca
      121 taacaacgtt caaatgcggc aacaaattcc ggactattcg gaattgcggt gccaaaaatt
      181 tcttcaactg ccagcagacg ctggctaatc agtgcatcat ctgaaaccag accctgacaa
      241 aattctgcac gcggatcagg aatggtatag ctcacaccat tttcatcaac gcctttcaga
      301 tacagtgccc atgctgcaac aaccagtgct gcacgttcgg tttcacgacc atctgcaatc
      361 agacgattaa ttgtcggaac ggtaaattta ggaaatttgc tgctaccatc actacaaaca
      421 cgttccagct gatctgcaat tgcctgattg ctaaaacgat caaccagggt ctgtttataa
      481 tcggtcagat caatacccgg aaccggagcc agattcgggg tcacatccag atccatatat
      541 gcacgcatat aggcaacaaa cagcggatcg ttcatggttt catgcacaaa acgataacct
      601 ttcagaaaac ccagataggt cagtgccagc gcgctaccat tcagcagacc aattttcatt
      661 tcttcatacg gggtaacatc atcggtaaac tgaacaccaa ctttttccca tgccggacga
      721 ccattaacaa atttatcttc cagaaccca
//
