LOCUS       sequence-123894-         531 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector; single gRNA under OsU6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ141C
FEATURES             Location/Qualifiers
     source          1..531
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..90
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_RNA        439..514
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt
       61 ttataatgcc aactttgtat acaaaagttg ccccatggcg ttccctctag ataacgcagg
      121 atccactagt aacggccgcc agtgtgctgg aattgccctt ggatcatgaa ccaacggcct
      181 ggctgtattt ggtggttgtg tagggagatg gggagaagaa aagcccgatt ctcttcgctg
      241 tgatgggctg gatgcatgcg ggggagcggg aggcccaagt acgtgcacgg tgagcggccc
      301 acagggcgag tgtgagcgcg agaggcggga ggaacagttt agtaccacat tgcccagcta
      361 actcgaacgc gaccaactta taaacccgcg cgctgtcgct tgtgtggaga cgagatctag
      421 tctgagtcga ccgtctctgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg
      481 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tgtcccttcg a
//
