LOCUS       sequence-123897-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector; single gRNA under OsU3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ141D
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..89
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        126..503
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     misc_RNA        536..611
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(700..799)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(817..835)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(852..870)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(875..891)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(875..891)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgtata caaaagttgc cccatggcgt tccctctaga taacgcagga
      121 tcctaaaggg atctttaaac atacgaacag atcacttaaa gttcttctga agcaacttaa
      181 agttatcagg catgcatgga tcttggagga atcagatgtg cagtcaggga ccatagcaca
      241 ggacaggcgt cttctactgg tgctaccagc aaatgctgga agccgggaac actgggtacg
      301 ttggaaacca cgtgatgtgg agtaagataa actgtaggag aaaagcattt cgtagtgggc
      361 catgaagcct ttcaggacat gtattgcagt atgggccggc ccattacgca attggacgac
      421 aacaaagact agtattagta ccacctcggc tatccacata gatcaaagct ggtttaaaag
      481 agttgtgcag atgatccgtg gcagagacga gatctagtct gagtcgaccg tctctgtttt
      541 agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac
      601 cgagtcggtg ctttttttgt cccttcgaag ggcaattctg cagatatcca tcacactggc
      661 ggccgctcga ggtcgagggt atcgataagc ttgaattcga cccagctttc ttgtacaaag
      721 ttggcattat aaaaaataat tgctcatcaa tttgttgcaa cgaacaggtc actatcagtc
      781 aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg
      841 tcatagcatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
      901 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaat
//
