LOCUS       sequence-123974-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector; 8th gRNA under AtU6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ138A
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(479..554)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(588..891)
                     /label=AtU6-1 promoter
                     /note="promoter for the Arabidopsis thaliana U6-1 snRNA 
                     gene (Waibel and Filipowicz, 1990)"
     primer_bind     980..996
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     980..996
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1001..1020
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1001..1019
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact
       61 cacgttaagg gattttggtc atgggtggct cgagggtctc ttcgagctga tcctaaatgc
      121 tatcaagttt attatatttt tgtgaaagaa atgttgtgtt tcaatctttg gtctatctaa
      181 tttcaccctt cataagaaaa tgatgtagcc gtctggaaac aaactctcga tacatttctt
      241 acccgaaatc tggttaacaa aacgcattgg tgtcgccgac tcgaaacaaa ctctctgaca
      301 atcagaactg aaacaacgga ccaatcactt tgtcttagct atctaaataa gctcattgca
      361 ggtccaactt ttttcgcgca gatttgcatc caaaacttca cttatgattc acgaaacgga
      421 aaaaaaaacc cagaaatcta cagatgaaga aaaaatctga aaatttttgc caaaaaaagc
      481 accgactcgg tgccactttt tcaagttgat aacggactag ccttatttta acttgctatt
      541 tctagctcta aaacagagac ggtcgactca gactagatct cgtctccaat cactacttcg
      601 tgtctaacca tatataaact cagctgcttt ctttacctaa gcgctgtggt acttttgaag
      661 attgttaata gcttaaatgg gcctatttta gaaaaaggcc tatccctcgc ttccttctca
      721 agcccattta tattattgaa ttttttccca actaaagatt ttattttctt atgcaagtct
      781 cactcacact cacgtaaatt tcaaatataa tcgtggtgga actaaaacaa tgaccgtctg
      841 gtttctacgg atcgagattc aaaattgttc tgccggaatt ttgagatttc ttttcggaga
      901 cctctagagg aggcatgatg atatattttt atcttgtgca atgtaacatc agagattttg
      961 agacacgggc cagagctgcc aggaaacagc tatgaccatg taatacgact cactataggg
     1021 ga
//
