LOCUS       sequence-123977-        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector; 1st gRNA under AtU3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ131B
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(349..368)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(350..368)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(373..389)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(373..389)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_RNA        837..912
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gctcgcggct cttaccagcc taacttcgat cattggaccg ctgatcgtca cggcgattta
       61 tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct
      121 tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga
      181 agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg
      241 cggagaactg tgaatgcgca aaccaaccct tgatcgggga agaacagtat gtcgagctat
      301 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatatccc ctatagtgag
      361 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
      421 tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaggtc tcgctatttt
      481 actttaaatt tttcttatgg ctcagcctgt gatggataac tgaatcaaac aaatggcgtc
      541 tgggtttaag aacatctgtt ttggctatgt tggacgaaac aagtgaactt ttaggatcaa
      601 cttccgttta tatacggagc ttatatcgag caataagata agtgggcttt ttatgtaatt
      661 taatgggcta tcgtccatat attcactaat acccatgccc agtacccatg tatgcgtttc
      721 atataagctc ctaatttctc ccacatcgct caaatctaaa caaatcttgt tgtatatata
      781 acactgaggg agcaccattg gtcagagacg agatctagtc tgagtcgacc gtctctgttt
      841 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
      901 ccgagtcggt gctttttttt ccctttcctt ttttcttttt tttgccataa acttaaattt
      961 gtatatcgat cattgtagat attgaaaacc tagaacaaac caacatccat gtgaatgtct
     1021 ttcatgactg atttagagat aattcttgaa ttttggaact agaatctata atg
//
