LOCUS       sequence-124028-        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with AteCas9 (Arabidopsis codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ154
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(13..136)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      complement(181..433)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(484..504)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcagctggat ggcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
       61 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcaact
      121 tttgtataca aagttgcgaa ttcgcccttt ccggaagctg tcgagggggg atcaattccc
      181 gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt
      241 tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca
      301 taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt
      361 atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa
      421 tgtttgaacg atcggacgtc accggtgttt aaacgctagc tctagagagc tcggcgcgcc
      481 tcaaaccttc ctcttcttct taggatcagc ccttgaatca ccaccgagct gtgagagatc
      541 gatcctagtc tcgtagagtc cagtgataga ctgatggatg agggtagcat cgagcacttc
      601 tttggtagag gtgtatctct tcctatcgat ggttgtatcg aagtacttga aagcagcagg
      661 agcaccgagg ttggtaaggg tgaagagatg gatgatgttc tctgcctgtt ccctgatagg
      721 cttatctctg tgcttgttgt aagcagacaa caccttatcg aggtttgcat cagcgaggat
      781 caccctttta gagaactcag agatctgctc gatgatctca tccaagtagt gcttgtgctg
      841 ctcaacgaaa agttgcttct gctcgttatc ttctggagat cccttcaact tctcgtagtg
      901 agaagcgagg taaagaaagt taacgtactt agatgggaga gcaagctcgt ttcccttttg
      961 aagctcacca gcagaagcga gcatcctctt tctaccgttc tcgagttcga agagtgagta
     1021 ctttgggagc ttgatgatga gatccttctt aacctctttg tatcccttag ccc
//
