LOCUS       sequence-124043-       16880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  attB flanked genomic Cirl for integration at attP sites. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16880)
  AUTHORS   Scholz N, Gehring J, Guan C, Ljaschenko D, Fischer R, Lakshmanan V, 
            Kittel RJ, Langenhan T
  TITLE     The adhesion GPCR latrophilin/CIRL shapes mechanosensation.
  JOURNAL   Cell Rep. 2015 May 12;11(6):866-74. doi: 
            10.1016/j.celrep.2015.04.008. Epub 2015 Apr 30.
  PUBMED    25937282
REFERENCE   2  (bases 1 to 16880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.04.008"; journalName: "Cell Rep"; date: 
            "2015-05-12- 12"; volume: "11"; issue: "6"; pages: "866-74"
COMMENT     Sequence Label: pTL370
FEATURES             Location/Qualifiers
     source          1..16880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    353..386
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     regulatory      2713..2722
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    11175..11208
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    join(14252..14806,14807..14837)
                     /label=P element 5' end
     rep_origin      complement(15072..15660)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15152..15171)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15831..16622,16623..16691))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16454..16473
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16692..16796)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagctag
      241 agttgttggt tggcacacca caaatatact gttgccgagc acaattccgc tcgaattgcc
      301 ggctgtacac ttaagaagtt cctactgcag gtaatgaatt gccgcggtgc gggtgccagg
      361 gcgtgccctt gggctccccg ggcgcgtact ccacgaattc ttgcatgcaa tgcggccgca
      421 gtaatttgtc ttcgatgtat gcataacaat caccacgtta cattcctatt tgaattgcgc
      481 gctcttccca gcagcaagtt ggcagtccga tagttgcgtt tttaggctgg caacaccctc
      541 cagcttcaac cagacattca agtatacaga atttttctaa aagaaagttg attgtgaatc
      601 gagctctcaa gcgaatagga tcgtctgcgt tgtatggagt ttaatccgaa atataagtgt
      661 aataattgcc caaagtgaag taaaacctag tgctaaccaa ggagcaaagc atccgtgaat
      721 aaaaaaaaac aaaaaagcaa cttaaagcca gtctttttcg tggctttggc atcatcaata
      781 actaagccaa caaatcgctc tgaaaatcgc caactggcga cagaaatcaa cgggaaaact
      841 tcattccgag tttaaaaatg ttttattctc gcggaattca agaaacgtcc acagttttca
      901 acgtgaaaaa aatgcccgga gttggaacaa agaaagacac ccaaactggg gctccgcggg
      961 gcagaaaaaa ctcgaagcgc agctgcagca acttttctct acatttacat ccacatgcat
     1021 atatgttcgt gaatagtgtg catcgggtgt acaaagaaaa cgggtctttg ttctgtccac
     1081 gaatcgatgg ccatgcaaca actacaacca cagtgtgggt gtacacagaa gggtgtgtat
     1141 tcttagctcc ctaccctacc ccctcccctg ccccatgcca tcttcttatc ttctatttgt
     1201 cttttgcctg cttgttcgtg tttgcccaag gagtcaatat gtaaacaatt atctaatgga
     1261 ttctctacct ttcagataca taagttgaat ggtacttttg agtccaactt tcatgaatat
     1321 gactccaaac ggaaatacat aagaggtatg tacatatgta cataggtgtg cttggcctgg
     1381 atatcatcat acataaatat aagaccattt taattattaa agatatataa atgcaattta
     1441 taattcttac aattaattat gttttcttga tatttgcatt gcttatatag tgttttaata
     1501 ggagttatac aaagaggttc tgaatattaa aataacttaa atttattaga atctacactt
     1561 ttgatattta ctgtaaccag gtcattagat tgactcaaca ttgatgtgat attgcacaag
     1621 taaattaaat ggaaccaata tcaataaaag aaaaggatag ttctcctttt cctttctaac
     1681 gaaatacaac atgtcttaag ttattagtta tgtgccttaa aataataata ataataatac
     1741 caaacattat aaatgttatc ttccctttca gtttgaatac ttcaaaccga gtgaaacact
     1801 gccgaatgag cattgccgat aagaaccaat gatggacgat cgatgattcg tgatctgggc
     1861 cggcaggatt tggtatttat gactactatt gcacttgcta ccaagctaac gacccacata
     1921 ctccaccatc aacctcagca ttactaagta tatcccaggc agtgtaaaag cgtatcatgc
     1981 taccaaccat attgtcaata tcctatgagc atatatcgtt agatcgtaag tgtctttgca
     2041 ggcccaccaa caatccaatc caaaccaaac caaactacaa caatcatcgc cgctaaccaa
     2101 aaaatttctt tttatatgcc atatatatat accatgtcca tataaaccca taccctgccc
     2161 agcaccctgt gacttatgaa gccttcgcct ttcagtgtcc aagtaccaaa ccgcctacgc
     2221 ctgcgaaggt aagaaactga ccatcgagtg cgatcccggc gatgtgatca acctcattcg
     2281 ggccaactat ggccgcttct cgattaccat ctgcaatgac cacgggaatg tggagtggag
     2341 tgttaactgc atgtttccca agtcactcag cgtactgaac tcaaggtaat acatctttta
     2401 aagtttattg gatcttcaaa tcattaaaca tgtcatttgg aaatttcata ctctggttgc
     2461 gaatatcaat aaactatttc aacacaagtc aatcaatcaa gcatcttaaa tatgtgaaaa
     2521 ttacccaact ctgtttactg atgaacgcgt atttttccca ttttccagat gtgcccacaa
     2581 gcagagctgc ggcgtgttgg cagccacgag catgttcggg gatccctgtc ccggtaccca
     2641 caagtatctg gaggcacact accagtgcat aagtgcagcc caaacttcga cgacgaccaa
     2701 caggcccagt ccgccgccat gggtgctgag caatggtccg ccgatctttg gcaacggcag
     2761 tggactgatc catccgcccg gggttggagc gggtgcgccg cccccgccga gacttcccac
     2821 acttcccgga gtggtgggaa tcagtgggaa tcccggcctg ttcaacgtac caccgcaaca
     2881 caccgccgtc acgcactcca cgccctcgag cagcacgaca gccgtgggcg gtggacgttt
     2941 gaagggtggg gccacctcca cgacgaccac caagcatccg gctggccgcc atgatggtct
     3001 gccaccgccg ccgcaactgc accaccacca caaccaccac ggtgaagaca ctgcctcacc
     3061 caccaagccg agcagcaagc tgccggctgg cggtaatgcc acttcaccat ccaacacgag
     3121 gatactcacg ggcgtcggag gttccggaac tgatgacgga accctactga ccacaaagag
     3181 ctcacccaac cgcccaccgg gcactgcggc cagtggatcc gttgtccccg ggaacggcag
     3241 cgtggtgcgc accatcaaca atattaattt gaacgcagcc gggatgtccg gaggcgatga
     3301 tgagtccaag ttgttttgcg gccccactca tgcccgcaat ttgtactgga acatgactcg
     3361 agtgggtgat gtgaatgttc agccctgtcc tggcggagca gccggcatcg ccaagtggcg
     3421 ttgcgttcta atgaagagga tacccgactc cggctacgat gagtacgatg atgacatcag
     3481 ttcgacaact ccggcaccca gcggtggcga ctgtctgcac aacagcagca gctgcgagcc
     3541 gccggtgagc atggcccaca aggtaaacca gcgtctgcgc aactttgagc ccacctggca
     3601 tcccgcgaca cctgatctga cgcaatgccg cagcctttgg ctcaacaatc tggaaatgcg
     3661 agtaaaccag cgggactcct ccttgatctc catcgccaac gatatgtccg aagtgaccag
     3721 tagcaaaacg ctctacggcg gcgacatgtt ggtcaccacg aagattatcc aaacagtgtc
     3781 cgagaagatg atgcacgaca aggagacctt cccggatcag cgacagcgcg aggctatgat
     3841 catggagttg ttgcattgtg tggtcaaaac cggctccaac ctgctggacg aatcgcagct
     3901 gtcctcgtgg ttggatctca atccggagga ccaaatgcgt gtagccacat ccttgctaac
     3961 tggcctggaa tacaatgcct ttctgctggc ggatacgatc atcagggagc gcagcgtggt
     4021 gcaaaaagtc aaaaatatat gtgagttcta aatgtttgca ttgtttgcat ttcaaaatac
     4081 ttataactgt atatcaccat agtgctctcc gttcgagttc tggaaaccaa gactatccag
     4141 tccagcgtgg tcttcccaga ttcggatcag tggcccttga gttcggatcg tattgagctg
     4201 ccacgagctg ctctaataga taatagtgaa ggcggtctgg tgcgaattgt attcgccgcc
     4261 ttcgatcgcc tggaatccat tctaaagccc agctatgatc acttcgatct caagagctcc
     4321 cgcagttacg gtaagtccac agagaactga aaaacgttag aatagtataa tctaaatgtt
     4381 ttcttagttc gcaatacagc catcctgagc aacgacagcg atgtcaacgc gggggagatc
     4441 caacagcgcc tacgcatcct gaacagcaag gtgatctcgg ccagcttggg caaggggcgt
     4501 cacatacaac tctcccagcc cataaccctg acactgaaac atctgaagac cgagaatgta
     4561 acgaatccca cctgcgtgtt ctggaactat attgaccagt aagactcata tgctcatatg
     4621 cctaataatt tatacgaatt aatgtttttg ttttagtgcg tggtctgcca acggatgcag
     4681 tctggagtcc actaaccgca cgcacagcgt ctgcagttgc aaccacctga caaactttgc
     4741 catactaatg gacgttgtgg atgagcacca gcattcgttg ttcaccatgt tcgatggaaa
     4801 catgcgcata ttcatctaca taagcatcgg catctgcgtg gtcttcatag ttatcgccct
     4861 gctaacgctg aagctgttca atggggtctt tgtgaaggta agaaacggct ccaatccctt
     4921 gccgcatcag cggtcgggca gcagacgcca gcaaaacaat attcgcgacc agacccacga
     4981 gtccttgacc ctgaccacgc caaccagtca gtccaatgtg ccgccgccca gtcatgggaa
     5041 cacgaacttt atccaacaca attccatccg caactcacac cgcaacaatc tgaactacaa
     5101 tgtccaacag cagcagcagc aacagcaaca acaagttgtt gcggcagctg ctgctgctgt
     5161 tgtgctgtcc aatcagccgc agcgaaatat gcaacatgcc atgaacaact tgaatctgaa
     5221 tctgcatcag cacggtcagc agacggctgc tgctgcagct gctgctgcct caatagccgc
     5281 tgcactgcag caacatgcag ttcaggccag caacgccagc aataacctca acatcagcca
     5341 caactatctg cagcagcagc atgtccagca gcagcagcag cagcagcgtg gccagcagcc
     5401 gcagccacat ccgcaccgca atcacaacct taatgtggac ggcaatggat tggataacac
     5461 gaacaacatc attatgcagg ccaacatgga cgacttacaa tataagtggt ggtggtggtg
     5521 aaaaaacaga taaacacaat tttaggctag gctaggcttt ctagacatat gtatttatga
     5581 ccgagagaca catgtgatat aatgcttaag taacttaaat tgttaatgaa cctctctctg
     5641 gcaggattca aagataagac tcaaaagctg tgctgggcag cgatgatcac tgttgcgtcc
     5701 cagtaacagc ttgcacgcga agactcccat ttatatttat gtatcgtagt aatttgtcat
     5761 agcttaagat tagcacacaa aaccaataat gaaatatctt catatgatag actcattttt
     5821 ctttttgtta caaatcatca ctctggcatg ttttacttaa tcgtttaatc atttttgaaa
     5881 taccataacc ccttttatgt gcattgattg tttaacaaat ttaacataga catcgtacaa
     5941 ctaaatacct agaacacttc tgactttctt ttgtatttca ttaattaata aaatatacaa
     6001 atgttgctaa ttttgtcctg taaatattgc attttgtgtt tgttcattta gtttgtatgt
     6061 atattaccta tgtgttgtgc ttgtttcgtg ttctgttcat tgttttattc gtattcgtat
     6121 tactcttagt ctttggattt tcaccaccac ccaccaaaat tattgtaaat caagttctaa
     6181 attaagtttg actatcaaat ctgacaccta ttcttcaaaa cgtttaagat tatcaatttc
     6241 cttaaaatgt ctaaaagctc cctgatgatc gaatctttag ggtccttctg ctggtgctgc
     6301 ttaaagaaaa aatttgcact gacttctcga acttatatta acttttcact ctgtcttctc
     6361 tttaactgtc cactccagtc cgcgcgcacc tcgatctata ccagcattta cctttgcctc
     6421 ctggccatcg agctgctctt tctcctgggc attgaacaga ccgaaacaag cattttctgc
     6481 ggcttcatta ctattttcct acactgtgcc atcctatcgg gcaccgcctg gttctgttac
     6541 gaaggtaaga aggccgaatt tcatctacac tttgcagccc taaccctttg cctctgcagc
     6601 cttccattcg tactcaacgc tcacctcgga cgagctcctg ctggaggtgg accagacgcc
     6661 caaggtgaac tgctactacc tcttgtccta cggactgtcg ctgagcgtgg tggccatctc
     6721 gctggtcatc gatcccagca cctataccca aaacgattat tgcgtgctga tggaggcgaa
     6781 tgccttgttt tatgccacct ttgtaatacc agtgcttgtc ttctttgtgg taagttacat
     6841 aatatgagat gaagcttcaa aagcgcagtt accattttaa attaattcac ttttacaggc
     6901 tgccattggt tacacattcc tctcctggat tataatgtgc cgcaaaagtc gcacgggtct
     6961 aaagaccaag gaacatactc gcctcgctag cgtgcggtaa gatattgaaa atccatatac
     7021 actttaaaga tccttcgatg cccatgtacc tcaaatccta ggttcgacat acgctgctcc
     7081 tttgtgttcc tcttgctgct cagcgctgtt tggtgctcgg cctacttcta tttgcgagga
     7141 gccaaaatgg acgatgacac ggctgatgtg tatggatact gcttcatctg cttcaacaca
     7201 ttgctggggc tctatatctt cgtgttccat tgcattcaaa acgaaaagat ccggcgggag
     7261 tatcggaagt atgtgagaca gcacgcttgg ctgcccaagt gcttgcgctg ctcgaaaaca
     7321 tcaatttcct cgggcattgt taccggcaat ggacccacag ccggaaccct ttgcagcgtc
     7381 tccacgtcca agaagcccaa gctgccgtta ggagtgagcg aagaggcgca tgacgatccc
     7441 cagcagcaac agcagacacc agtgcccatc acagaggatg ccattatggg agccacctct
     7501 gattgtgaac tgaacgaggc ccagcaaaga agaaccctaa aaagtggcct aatgacgggc
     7561 acactacagg ctccaccgca gacccttggt ggccatgttg tgctcgaaag aggtagcact
     7621 ctccgctcca ctggtcatgc ctcacccacc agctctgccg ggtccacaca cctgattttt
     7681 gcgcacaagc aacaacaaca acagcagcaa cagggacctt tgggcgagtc ttactaccat
     7741 cagccggact actacagctg gaagcaacca tcaactggaa caggaggatt gaaaacaccg
     7801 cgggagtact acaataatgc gggtgctgct gcatcatcgc cgcaacaggc gcacgaggta
     7861 ttctactgga ctcaaaagcc gaacagcggc cacaatggca aaaagaagag gggcgccgga
     7921 ggagttcccg cctcgcctag tggatctctg cacagtcgca cggccgccgc ctcccaggtg
     7981 cttttctatc catcgtacaa gaagaccaag cctggccagc caacaggcta tccgcaatac
     8041 gcggaggcgt tggacccacc actagccact ggcaatgcgg ctgcctacta ccagcagcag
     8101 caacagttgc gtcgccagca gctacatcag cagcagcaac agcagcagca gcagcaactc
     8161 tcctcggacg aggagcaggc cgagcaacat gctcacctgt tgcacctgca acgacgagct
     8221 ggtagccagc agcagctccc tgctccaccg ccacacatgg cgcagtacca gcaggagttt
     8281 atgcagcgcc agtatagaaa taagcattcc aactgtgatc tgggcatggg cgatgcctac
     8341 tacaaccaag gcagcgtcgg cggcgcggat ggtgggccgg tctacgagga gatcctcagc
     8401 aaccgcaact cggatgtgca gcattacgag gtgggtgact tcgatgtgga cgaggtgtac
     8461 aacaatagcg ttggcactgg cgtcttcaac aacatgagag cggcggtggc cgccggcggt
     8521 agtcggtacg gcggcggaag cctgagtggc ggcagtgtct cgtccaggag ccaacagcag
     8581 cagctcaaga agcagcagca acaacagtcg ctggctcagc aaagatcggc tcgacgctgc
     8641 acggcggatg acgatgacga cgaagacgag gaggaggatg aggaggcaac ggccgcggag
     8701 caattgcacg acagcgtctg tgatgaggat gaggaggagg acgagagcga cttggaggat
     8761 gatgctcatg gattgccacc gcagagcgat gagcggatgc gtcgtctgat ggcgatgcag
     8821 gacgaggatt ttaagcggcg gtttcagtaa gtacttctaa tgtttgacta tatcaacgtt
     8881 acgaaaaaat tacatttatc ccctttacag acgtcagctg cgcaaacatg gagcgcctct
     8941 tgactacggg gctttgccac caggagcagg accgcaaccc gagcacaacg gtgcggtttt
     9001 tggggttagc ggcggcgttg gtgagggctc cctgcgtggc gcattccggc agcagcagca
     9061 gcaagcactg aatgccaagt cgccaggcgg ccgtttggcg gtaaatgagc tattcggtca
     9121 cggcaactcg ggaccaccgc tgccgccggc gaatcagacg cccgcgcaga agcgccagca
     9181 gctacagaaa ctgtcaccac agtccaccac atcatcgtcg tcgcatacat cacacagcaa
     9241 tcccaatccg catccccacc agctaaccca tccgcatccc catcagcatc ctccgcacca
     9301 tcagcagcgc cacctgtcgg ccatgctcga tgagaacaac acggtgcgat gttatctgga
     9361 accactggct aagtgatcag ttaacgcaac tcccccggaa cagtcttagg caaaaattgt
     9421 agcttaggat ctctagctat acctagtatt agtcgttagt cgaaatatgg agcattgtgt
     9481 gaaacaacat gctgtcatgc tatacgtgca gtgattcgaa aatcccaaaa ctttataaat
     9541 gtatatgaaa taactaaaac ctagaccata gggcacatcc atacccctgc ggtgccgtgc
     9601 aagcttaacc aaaactcgag cgatgggggg ttggaattgg aattggaatc gggcccttcg
     9661 acttgcatgc attttatacc aacttggaaa tcgatattaa atataaatgt atgtaccata
     9721 tatgtatgat gtattgtaat acatgtcacc acatgccatt tttaaatact taaaaactaa
     9781 tttaaacaac cagaaaatca agtatatata tatggatgta ggtatatgta taagatctag
     9841 gaactgtatg tacatgatat atagaattca ggcgaattat gttttatgta ttttgtaaga
     9901 aatcaattgt ttttgattta cgctgattat gctcgtaaga tatatatata tatacacaca
     9961 ggcttaagat ttgttttgtg tttcataaac caaaaagata tcctacgttt tgaaagattg
    10021 tcatttcttg ctatccagct gccgttttcc acctatacaa ctaaaattcc ttccaactgc
    10081 gaatcaagag gaatataaga agtaaaggga aataatacat tgccattttg ttgctgcatt
    10141 tcagctacgt tgtaagcaaa ccagagcgaa gcatttgaag tgtacatttt catcaatcta
    10201 gatgctttaa gcacacacat aggggcattt actttatatt tttgagatac agagagagag
    10261 agagctcgcg aaatgcttta ttattttaca aaaccgatac agcatacgga aatgaaatac
    10321 ctatatagca aagaaagatt tcaaccaaac ctcacttgtt aactaggaac taactgaaaa
    10381 ctctttaaac actagccagc tgaaatccaa tcctaacact aaactgtact ctattcctac
    10441 tccccgaatg gcattctccc tagaatttca cgcatgtgta caaaaaccaa ataaaatata
    10501 gtgcaaacaa gatgattacc atatatgtcg tacataccaa gccaaggaag ctccgtgggg
    10561 gcagcaggcc cagctcgatc ccatttgatc tgatccgatt cgatccgatc cgatcagcaa
    10621 gttaaacttg aacgaaacat tagcacacaa tcgataaagt tagtaactaa gcacccgcag
    10681 catgccacac ataaagtgtc taaattggtt cggaaatcgc gtgtttgaaa gaggaataca
    10741 gaatagcaga tattgaatgt atagtgttgc gcctcctaga actttactta taaataataa
    10801 agcagattta tcagatacga gtatatatgt gcgtgtatat tgacgatgca agccattgca
    10861 atccccctct tttgtcgcca cattctcgcg aaattgattc tagacctagg cattgaattg
    10921 ataaatcaga gattttgaat aaagcagaaa ccgaaactga aaaccagacg cccaaatcaa
    10981 agttcataaa aagcccgatg aaaatgttaa tgtacacaat tttttctatt taagacaaaa
    11041 tacaagaaat ggattgcatt ttactgtaaa tgacaaataa gtgttttctt gggatattgg
    11101 gttcaaaatt taaaacgggt gtttaattta aatagtttgc tttcaaaaat ggctttaaat
    11161 ggcgcgccgg taccataact tcgtataatg tatgctatac gaagttatcg tacgactagc
    11221 acctgactgt cgagcccagt ggaaaccctt gaaatgcctt taagtcgagc ccagtggaaa
    11281 cccttgaaat gcctttaagt cgagcccagt ggaaaccctt gaaatgcctt taagtcgagc
    11341 ccagtggaaa cccttgaaat gcctttaagt cgagcccagt ggaaaccctt gaaatgcctt
    11401 taagtcgagt agcagtcgag gtaggtacgc catgccccgt tattctctat tcgttttgtg
    11461 actctccctc tctgtactat tgctctctca ctctgtcgca cagtaaacgg cacgctattc
    11521 tcgttgcttc gagagagcgc gcctcgaatg ttcgcgaaaa gagcgccgga gtataaatag
    11581 aggcgcttcg tcgacggagc gtcaattcaa ttcaaacaag caaagtgaac acatcgcgaa
    11641 gcaagctgag caaacaaaca agcgcagctg aacaagctaa acaatctgca ataaagtgca
    11701 agttaaagtg aatcaattaa aagtaaccaa caaccaagta attaaactaa aaactgcaac
    11761 tactgaaatc aaccaagaag tcattattga agacaagaag agaactctga ataggtcgat
    11821 agcgtcaatt cggcacgagg tttcgtgacg aagctccaag cggtttacgc catcaattaa
    11881 acacaaagtg ctgtgccaaa actcctctcg cttcttattt ttgtttgttt tttgagtgat
    11941 tgggtgggtg attggttttg ggtgggtaag caggggaaag tgtgaaaaat cccggcaatg
    12001 ggccaagagg atcaggagct attaattcgc ggaggcagca aacacccatc tgccgagcat
    12061 ctgaacaatg gtgacagcgg agcggcttcg cagagctgca ttaaccaggg cttcgggcag
    12121 gccaaaaact acggcacgct cctgccaccc agtccgccgg aggactccgg ttcagggagc
    12181 ggccaactag ccgagaacct cacctatgcc tggcacaata tggacatctt tggggcggtc
    12241 aatcagccgg gctccggatg gcggcagctg gtcaaccgga cacgcggact attctgcaac
    12301 gagcgacaca taccggcgcc caggaaacat ttgctcaaga acgtttgcgg cgtggcctat
    12361 ccgggcgaac ttttggccgt gatgggcagt tccggtgccg gaaagacgac cctgctgaat
    12421 gcccttgcct ttcgatcgcc gcagggcatc caagtatcgc catccgggat gcgactgctc
    12481 aatggccaac ctgtggacgc caaggagatg caggccaggt gcgcctatgt ccagcaggat
    12541 gacctcttta tcggctccct aacggccagg gaacacctga ttttccaggc catggtgcgg
    12601 atgccacgac atctgaccta tcggcagcga gtggcccgcg tggatcaggt gatccaggag
    12661 ctttcgctca gcaaatgtca gcacacgatc atcggtgtgc ccggcagggt gaaaggtctg
    12721 tccggcggag aaaggaagcg tctggcattc gcctccgagg cactaaccga tccgccgctt
    12781 ctgatctgcg atgagcccac ctccggactg gactcattta ccgcccacag cgtcgtccag
    12841 gtgctgaaga agctgtcgca gaagggcaag accgtcatcc tgaccattca tcagccgtct
    12901 tccgagctgt ttgagctctt tgacaagatc cttctgatgg ccgagggcag ggtagctttc
    12961 ttgggcactc ccagcgaagc cgtcgacttc ttttcctacg tgggtgccca gtgtcctacc
    13021 aactacaatc cggcggactt ttacgtacag gtgttggccg ttgtgcccgg acgggagatc
    13081 gagtcccgtg atcggatcgc caagatatgc gacaattttg ccattagcaa agtagcccgg
    13141 gatatggagc agttgttggc caccaaaaat ttggagaagc cactggagca gccggagaat
    13201 gggtacacct acaaggccac ctggttcatg cagttccggg cggtcctgtg gcgatcctgg
    13261 ctgtcggtgc tcaaggaacc actcctcgta aaagtgcgac ttattcagac aacgatggtt
    13321 gccatcttga ttggcctcat ctttttgggc caacaactca cgcaagtggg cgtgatgaat
    13381 atcaacggag ccatcttcct cttcctgacc aacatgacct ttcaaaacgt ctttgccacg
    13441 ataaatgtgt tcacctcaga gctgccagtt tttatgaggg aggcccgaag tcgactttat
    13501 cgctgtgaca catactttct gggcaaaacg attgccgaat tgccgctttt tctcacagtg
    13561 ccactggtct tcacggcgat tgcctatccg atgatcggac tgcgggccgg agtgctgcac
    13621 ttcttcaact gcctggcgct ggtcactctg gtggccaatg tgtcaacgtc cttcggatat
    13681 ctaatatcct gcgccagctc ctcgacctcg atggcgctgt ctgtgggtcc gccggttatc
    13741 ataccattcc tgctctttgg cggcttcttc ttgaactcgg gctcggtgcc agtatacctc
    13801 aaatggttgt cgtacctctc atggttccgt tacgccaacg agggtctgct gattaaccaa
    13861 tgggcggacg tggagccggg cgaaattagc tgcacatcgt cgaacaccac gtgccccagt
    13921 tcgggcaagg tcatcctgga gacgcttaac ttctccgccg ccgatctgcc gctggactac
    13981 gtgggtctgg ccattctcat cgtgagcttc cgggtgctcg catatctggc tctaagactt
    14041 cgggcccgac gcaaggagta gccgacatat atccgaaata actgtttgtt tttttttttt
    14101 accattatta ccatcgtgtt tactgtttat tgccccctca aaaagctaat gtaattatat
    14161 ttgtgccaat aaaaacaaga tatgacctat agaatacaaa aaaaaaaaaa aaaaaaaaaa
    14221 aaaaactcga caccgggatc caaacctagt ggctgctgct ctaaacgacg catttcgtac
    14281 tccaaagtac gaattttttc cctcaagctc ttattttcat taaacaatga acaggaccta
    14341 acgcacagtc acgttattgt ttacataaat gatttttttt actattcaaa cttactctgt
    14401 ttgtgtactc ccactggtat agccttcttt tatcttttct ggttcaggct ctatcacttt
    14461 actaggtacg gcatctgcgt tgagtcgcct ccttttaaat gtctgacctt ttgcaggtgc
    14521 agccttccac tgcgaatcat taaagtgggt atcacaaatt tgggagtttt caccaaggct
    14581 gcacccaagg ctctgctccc acaattttct cttaatagca cacttcggca cgtgaattaa
    14641 ttttactcca gtcacagctt tgcagcaaaa tttgcaatat ttcatttttt tttattccac
    14701 gtaagggtta atgttttcaa aaaaaaattc gtccgcacac aacctttcct ctcaacaagc
    14761 aaacgtgcac tgaatttaag tgtatacttc ggtaagcttc ggctatcgac gggaccacct
    14821 tatgttattt catcatgggc cagacccacg tagtccagcg gcagatcggc ggcggagaag
    14881 ttaagcgtct ccaggatgac cttgcccgaa ctggggcacg tggtgttcga cgatgtgcag
    14941 ctaatttcgc ccggctccac gtccgcccat tggttaatca gcagaccctc gttggcgtaa
    15001 cggaaccatg agaggtacga caaccatttg aggtatactg gcaccgagcc cgagttcaag
    15061 aagaaggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    15121 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    15181 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    15241 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    15301 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    15361 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    15421 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    15481 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
    15541 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    15601 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    15661 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    15721 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    15781 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    15841 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    15901 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    15961 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    16021 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    16081 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    16141 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    16201 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    16261 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    16321 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    16381 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    16441 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    16501 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    16561 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    16621 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    16681 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    16741 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    16801 tttccccgaa aagtgccacc tgcgaagtct tagagccaga tatgcgagca cccggaagct
    16861 cacgatgaga atggccagac
//
