LOCUS       sequence-124120-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    53..105
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      609..618
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(645..744)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(674..694)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 catgttctcc ttgatcagct cctcgccctt gctcaccatg gtggcgaccg gtggatcccg
       61 ggcccgcggt accgtcgact gcagaattcg aagcttgagc tcgagagtgc tcccgccact
      121 gccctccgag ggcaccgcgg ccggactggc aggcgccgcc ggggtgcggg gggcagcgtt
      181 tcgcgcgcgg gcgcgggcca cgccgctgca ggacaccacg ccgtggcgcc ggcggaagcg
      241 gtccagccag ccgttggagg cggtgaagtc gtccatgccc agctcctcgg ctatgcgcag
      301 cgccttctcc ttgaggatga tgcccttgac cggcaggccg gcggcgcgga tctgctggaa
      361 ccaggcgatg agcaagccct cgagcttgtc gtagggagac agcttgttgg tcttgcggca
      421 ggtggaggcc accccgtact tgcgctccga cgccaggatg gcgcgcttgt tcttcaggat
      481 cgtgctcagc gtggacggcg ggatgttgaa gcgccgcgcg atctcgccct tgcgtaggtc
      541 cgggttctcc tccacctcct ggatgatccg cgacttctcc cggaacgtca gctgccgccg
      601 cttggggccc atggtggcta gcggatctga cggttcacta aaccagctct gcttatatag
      661 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat
      721 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
      781 agacttggaa atccccgtga
//
