LOCUS       sequence-124147-         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-inducible RRNA promoter driven plasmid for the generation of 
            stem loop RNAi transcripts from a specific RRNA spacer locus in T. 
            brucei..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Alsford S, Horn D
  TITLE     Single-locus targeting constructs for reliable regulated RNAi and 
            transgene expression in Trypanosoma brucei.
  JOURNAL   Mol Biochem Parasitol. 2008 Sep;161(1):76-9. doi: 
            10.1016/j.molbiopara.2008.05.006. Epub 2008 Jun 7.
  PUBMED    18588918
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molbiopara.2008.05.006"; journalName: "Mol Biochem 
            Parasitol"; date: "2008-09"; volume: "161"; issue: "1"; pages: 
            "76-9"
COMMENT     Sequence Label: pRPa-iSL(MCS3+4)
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(181..202)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     393..412
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tacaacacac aatagggata ataatgataa agttaaaaaa gtatatatag taatagaaat
       61 atatcttata taggaaagat taagcagtaa aagtagcgct tacggcgtac accctatcaa
      121 tgatagagtg gtaccggtat cgatcaattg cccgggtccg agatagggtt gagtgttgtt
      181 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
      241 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
      301 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
      361 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
      421 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
      481 gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg gaagggcgat
      541 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
      601 taagttgggt aacgccaggg ttttcagatc ttaaggccgg cctcgagtct agagggcccc
      661 tgcaggaatt cgatgcgagg cgaatcgctc agtagtgtta gtgcaggcat tgtttctctt
      721 ttccatttcg gctcgctctc tgtgccagaa aggcttttgg agtcttggtt actcgtggga
      781 ctagggcaag
//
