LOCUS       sequence-124321-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  construction of ycrtI gene in HCKan-O receiving vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: HCKan-O-ycrtI
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(44..146)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(147..178)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 atcgcggcct cgagcaagac gtttcccgtt gaatatggct cattttagct tccttagctc
       61 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
      121 agttggaacc tcttacgtgc cgatcaagtc aaaagcctcc ggtcggaggc ttttgacttt
      181 ctgctatgga ggtcaggtat gatttaaatg gtcagtcgtc tcggctagaa agccaaaaca
      241 ccaacagatc gagcgataac agcaccgatc aacaaaacca acaagtaaac ccatctttcc
      301 aagtccatga acttcaattg aacttgagag tcgataccgg ttccgttaga cttcaatggc
      361 ttaccgtatg gaacagacaa agacatgttt gggtctggag ctggagatcg gtcgaaagat
      421 tccaaaactt ggttagcggt caacttagca ccagccaaaa cgattggaac accggttcct
      481 gggtgggtag aagcaccaac gaagaacaac ttgtcgtact ttgggtgtct ggtagatggt
      541 ctgaaaccca aaacttgcat gaagttgtga gccaaaccca agatagaacc gtccttcaag
      601 ttgaattcct tttcccaaga tggagcgtcg tgaacctttt cagcaacgat catcttttcg
      661 aagtctggca aacccaactt agcagacaag gtttggataa cgaactttct agctctagca
      721 accaacttgt tgtagtcttg tgggttagaa gcgtcgatgt gaccacatgg aaccaagata
      781 acgatagcgt ccttaccttc tggagcagca gatgggtcga ttctacttgg aacgttaacg
      841 tagaaagatg g
//
