LOCUS       sequence-124368-        3756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized Lb2Cpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY011 (pcDNA3.1-hLb2Cpf1)
FEATURES             Location/Qualifiers
     source          1..3756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3619..3666
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3673..3753
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 atgtactatg agtccctgac caagcagtac cccgtgtcta agacaatccg gaatgagctg
       61 atccctatcg gcaagacact ggataacatc cgccagaaca atatcctgga gagcgacgtg
      121 aagcggaagc agaactacga gcacgtgaag ggcatcctgg atgagtatca caagcagctg
      181 atcaacgagg ccctggacaa ttgcaccctg ccatccctga agatcgccgc cgagatctac
      241 ctgaagaatc agaaggaggt gtctgacaga gaggatttca acaagacaca ggacctgctg
      301 aggaaggagg tggtggagaa gctgaaggcc cacgagaact ttaccaagat cggcaagaag
      361 gacatcctgg atctgctgga gaagctgcct tccatctctg aggacgatta caatgccctg
      421 gagagcttcc gcaactttta cacctatttc acatcctaca acaaggtgcg ggagaatctg
      481 tattctgata aggagaagag ctccacagtg gcctacagac tgatcaacga gaatttccca
      541 aagtttctgg acaatgtgaa gagctatagg tttgtgaaaa ccgcaggcat cctggcagat
      601 ggcctgggag aggaggagca ggactccctg ttcatcgtgg agacattcaa caagaccctg
      661 acacaggacg gcatcgatac ctacaattct caagtgggca agatcaactc tagcatcaat
      721 ctgtataacc agaagaatca gaaggccaat ggcttcagaa agatccccaa gatgaagatg
      781 ctgtataagc agatcctgtc cgatagggag gagtctttca tcgacgagtt tcagagcgat
      841 gaggtgctga tcgacaacgt ggagtcttat ggcagcgtgc tgatcgagtc tctgaagtcc
      901 tctaaggtga gcgccttctt tgatgccctg agagagtcta agggcaagaa cgtgtacgtg
      961 aagaatgacc tggccaagac agccatgagc aacatcgtgt tcgagaattg gaggaccttt
     1021 gacgatctgc tgaaccagga gtacgacctg gccaacgaga acaagaagaa ggacgataag
     1081 tatttcgaga agcgccagaa ggagctgaag aagaataaga gctactccct ggagcacctg
     1141 tgcaacctgt ccgaggattc ttgtaacctg atcgagaatt atatccacca gatctccgac
     1201 gatatcgaga atatcatcat caacaatgag acattcctgc gcatcgtgat caatgagcac
     1261 gacaggtccc gcaagctggc caagaaccgg aaggccgtga aggccatcaa ggactttctg
     1321 gattctatca aggtgctgga gcgggagctg aagctgatca acagctccgg ccaggagctg
     1381 gagaaggatc tgatcgtgta ctctgcccac gaggagctgc tggtggagct gaagcaggtg
     1441 gacagcctgt ataacatgac cagaaattat ctgacaaaga agcctttctc taccgagaag
     1501 gtgaagctga actttaatcg cagcacactg ctgaacggct gggatcggaa taaggagaca
     1561 gacaacctgg gcgtgctgct gctgaaggac ggcaagtact atctgggcat catgaacaca
     1621 agcgccaata aggccttcgt gaatccccct gtggccaaga ccgagaaggt gtttaagaag
     1681 gtggattaca agctgctgcc agtgcccaac cagatgctgc caaaggtgtt ctttgccaag
     1741 agcaatatcg acttctataa cccctctagc gagatctact ccaattataa gaagggcacc
     1801 cacaagaagg gcaatatgtt ttccctggag gattgtcaca acctgatcga cttctttaag
     1861 gagtctatca gcaagcacga ggactggagc aagttcggct ttaagttcag cgatacagcc
     1921 tcctacaacg acatctccga gttctatcgc gaggtggaga agcagggcta caagctgacc
     1981 tatacagaca tcgatgagac atacatcaat gatctgatcg agcggaacga gctgtacctg
     2041 ttccagatct ataataagga ctttagcatg tactccaagg gcaagctgaa cctgcacaca
     2101 ctgtatttca tgatgctgtt tgatcagcgc aatatcgacg acgtggtgta taagctgaac
     2161 ggagaggcag aggtgttcta taggccagcc tccatctctg aggacgagct gatcatccac
     2221 aaggccggcg aggagatcaa gaacaagaat cctaaccggg ccagaaccaa ggagacaagc
     2281 accttcagct acgacatcgt gaaggataag cggtatagca aggataagtt taccctgcac
     2341 atccccatca caatgaactt cggcgtggat gaggtgaagc ggttcaacga cgccgtgaac
     2401 agcgccatcc ggatcgatga gaatgtgaac gtgatcggca tcgaccgggg cgagagaaat
     2461 ctgctgtacg tggtggtcat cgactctaag ggcaacatcc tggagcagat ctccctgaac
     2521 tctatcatca ataaggagta cgacatcgag acagattatc acgcactgct ggatgagagg
     2581 gagggcggca gagataaggc ccggaaggac tggaacaccg tggagaatat cagggacctg
     2641 aaggccggct acctgagcca ggtggtgaac gtggtggcca agctggtgct gaagtataat
     2701 gccatcatct gcctggagga cctgaacttt ggcttcaaga ggggccgcca gaaggtggag
     2761 aagcaggtgt accagaagtt cgagaagatg ctgatcgata agctgaatta cctggtcatc
     2821 gacaagagcc gcgagcagac atcccctaag gagctgggag gcgccctgaa cgcactgcag
     2881 ctgacctcta agttcaagag ctttaaggag ctgggcaagc agtccggcgt gatctactat
     2941 gtgcctgcct acctgacctc taagatcgat ccaaccacag gcttcgccaa tctgttttat
     3001 atgaagtgtg agaacgtgga gaagtccaag agattctttg acggctttga tttcatcagg
     3061 ttcaacgccc tggagaacgt gttcgagttc ggctttgact accggagctt cacccagagg
     3121 gcctgcggca tcaattccaa gtggaccgtg tgcaccaacg gcgagcgcat catcaagtat
     3181 cggaatccag ataagaacaa tatgttcgac gagaaggtgg tggtggtgac cgatgagatg
     3241 aagaacctgt ttgagcagta caagatcccc tatgaggatg gcagaaatgt gaaggacatg
     3301 atcatcagca acgaggaggc cgagttctac cggagactgt ataggctgct gcagcagacc
     3361 ctgcagatga gaaacagcac ctccgacggc acaagggatt acatcatctc ccctgtgaag
     3421 aataagagag aggcctactt caacagcgag ctgtccgacg gctctgtgcc aaaggacgcc
     3481 gatgccaacg gcgcctacaa tatcgccaga aagggcctgt gggtgctgga gcagatcagg
     3541 cagaagagcg agggcgagaa gatcaatctg gccatgacca acgccgagtg gctggagtat
     3601 gcccagacac acctgctgaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag
     3661 aaaaagggat cctacccata cgatgttcca gattacgctt atccctacga cgtgcctgat
     3721 tatgcatacc catatgatgt ccccgactat gcctaa
//
