LOCUS       sequence-124371-        4257 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized MbCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY014 (pcDNA3.1-hMbCpf1)
FEATURES             Location/Qualifiers
     source          1..4257
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4120..4167
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4174..4254
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 atgctgttcc aggactttac ccacctgtat ccactgtcca agacagtgag atttgagctg
       61 aagcccatcg ataggaccct ggagcacatc cacgccaaga acttcctgtc tcaggacgag
      121 acaatggccg atatgcacca gaaggtgaaa gtgatcctgg acgattacca ccgcgacttc
      181 atcgccgata tgatgggcga ggtgaagctg accaagctgg ccgagttcta tgacgtgtac
      241 ctgaagtttc ggaagaaccc aaaggacgat gagctgcaga agcagctgaa ggatctgcag
      301 gccgtgctga gaaaggagat cgtgaagccc atcggcaatg gcggcaagta taaggccggc
      361 tacgacaggc tgttcggcgc caagctgttt aaggacggca aggagctggg cgatctggcc
      421 aagttcgtga tcgcacagga gggagagagc tccccaaagc tggcccacct ggcccacttc
      481 gagaagtttt ccacctattt cacaggcttt cacgataacc ggaagaatat gtattctgac
      541 gaggataagc acaccgccat cgcctaccgc ctgatccacg agaacctgcc ccggtttatc
      601 gacaatctgc agatcctgac cacaatcaag cagaagcact ctgccctgta cgatcagatc
      661 atcaacgagc tgaccgccag cggcctggac gtgtctctgg ccagccacct ggatggctat
      721 cacaagctgc tgacacagga gggcatcacc gcctacaata cactgctggg aggaatctcc
      781 ggagaggcag gctctcctaa gatccagggc atcaacgagc tgatcaattc tcaccacaac
      841 cagcactgcc acaagagcga gagaatcgcc aagctgaggc cactgcacaa gcagatcctg
      901 tccgacggca tgagcgtgtc cttcctgccc tctaagtttg ccgacgatag cgagatgtgc
      961 caggccgtga acgagttcta tcgccactac gccgacgtgt tcgccaaggt gcagagcctg
     1021 ttcgacggct ttgacgatca ccagaaggat ggcatctacg tggagcacaa gaacctgaat
     1081 gagctgtcca agcaggcctt cggcgacttt gcactgctgg gacgcgtgct ggacggatac
     1141 tatgtggatg tggtgaatcc agagttcaac gagcggtttg ccaaggccaa gaccgacaat
     1201 gccaaggcca agctgacaaa ggagaaggat aagttcatca agggcgtgca ctccctggcc
     1261 tctctggagc aggccatcga gcactatacc gcaaggcacg acgatgagag cgtgcaggca
     1321 ggcaagctgg gacagtactt caagcacggc ctggccggag tggacaaccc catccagaag
     1381 atccacaaca atcacagcac catcaagggc tttctggaga gggagcgccc tgcaggagag
     1441 agagccctgc caaagatcaa gtccggcaag aatcctgaga tgacacagct gaggcagctg
     1501 aaggagctgc tggataacgc cctgaatgtg gcccacttcg ccaagctgct gaccacaaag
     1561 accacactgg acaatcagga tggcaacttc tatggcgagt ttggcgtgct gtacgacgag
     1621 ctggccaaga tccccaccct gtataacaag gtgagagatt acctgagcca gaagcctttc
     1681 tccaccgaga agtacaagct gaactttggc aatccaacac tgctgaatgg ctgggacctg
     1741 aacaaggaga aggataattt cggcgtgatc ctgcagaagg acggctgcta ctatctggcc
     1801 ctgctggaca aggcccacaa gaaggtgttt gataacgccc ctaatacagg caagagcatc
     1861 tatcagaaga tgatctataa gtacctggag gtgaggaagc agttccccaa ggtgttcttt
     1921 tccaaggagg ccatcgccat caactaccac ccttctaagg agctggtgga gatcaaggac
     1981 aagggccggc agagatccga cgatgagcgc ctgaagctgt atcggtttat cctggagtgt
     2041 ctgaagatcc accctaagta cgataagaag ttcgagggcg ccatcggcga catccagctg
     2101 tttaagaagg ataagaaggg cagagaggtg ccaatcagcg agaaggacct gttcgataag
     2161 atcaacggca tcttttctag caagcctaag ctggagatgg aggacttctt tatcggcgag
     2221 ttcaagaggt ataacccaag ccaggacctg gtggatcagt ataatatcta caagaagatc
     2281 gactccaacg ataatcgcaa gaaggagaat ttctacaaca atcaccccaa gtttaagaag
     2341 gatctggtgc ggtactatta cgagtctatg tgcaagcacg aggagtggga ggagagcttc
     2401 gagttttcca agaagctgca ggacatcggc tgttacgtgg atgtgaacga gctgtttacc
     2461 gagatcgaga cacggagact gaattataag atctccttct gcaacatcaa tgccgactac
     2521 atcgatgagc tggtggagca gggccagctg tatctgttcc agatctacaa caaggacttt
     2581 tccccaaagg cccacggcaa gcccaatctg cacaccctgt acttcaaggc cctgttttct
     2641 gaggacaacc tggccgatcc tatctataag ctgaatggcg aggcccagat cttctacaga
     2701 aaggcctccc tggacatgaa cgagacaaca atccacaggg ccggcgaggt gctggagaac
     2761 aagaatcccg ataatcctaa gaagagacag ttcgtgtacg acatcatcaa ggataagagg
     2821 tacacacagg acaagttcat gctgcacgtg ccaatcacca tgaactttgg cgtgcagggc
     2881 atgacaatca aggagttcaa taagaaggtg aaccagtcta tccagcagta tgacgaggtg
     2941 aacgtgatcg gcatcgatcg gggcgagaga cacctgctgt acctgaccgt gatcaatagc
     3001 aagggcgaga tcctggagca gtgttccctg aacgacatca ccacagcctc tgccaatggc
     3061 acacagatga ccacacctta ccacaagatc ctggataaga gggagatcga gcgcctgaac
     3121 gcccgggtgg gatggggcga gatcgagaca atcaaggagc tgaagtctgg ctatctgagc
     3181 cacgtggtgc accagatcag ccagctgatg ctgaagtaca acgccatcgt ggtgctggag
     3241 gacctgaatt tcggctttaa gaggggccgc tttaaggtgg agaagcagat ctatcagaac
     3301 ttcgagaatg ccctgatcaa gaagctgaac cacctggtgc tgaaggacaa ggccgacgat
     3361 gagatcggct cttacaagaa tgccctgcag ctgaccaaca atttcacaga tctgaagagc
     3421 atcggcaagc agaccggctt cctgttttat gtgcccgcct ggaacacctc taagatcgac
     3481 cctgagacag gctttgtgga tctgctgaag ccaagatacg agaacatcgc ccagagccag
     3541 gccttctttg gcaagttcga caagatctgc tataatgccg acaaggatta cttcgagttt
     3601 cacatcgact acgccaagtt taccgataag gccaagaata gccgccagat ctggacaatc
     3661 tgttcccacg gcgacaagcg gtacgtgtac gataagacag ccaaccagaa taagggcgcc
     3721 gccaagggca tcaacgtgaa tgatgagctg aagtccctgt tcgcccgcca ccacatcaac
     3781 gagaagcagc ccaacctggt catggacatc tgccagaaca atgataagga gtttcacaag
     3841 tctctgatgt acctgctgaa aaccctgctg gccctgcggt acagcaacgc ctcctctgac
     3901 gaggatttca tcctgtcccc cgtggcaaac gacgagggcg tgttctttaa tagcgccctg
     3961 gccgacgata cacagcctca gaatgccgat gccaacggcg cctaccacat cgccctgaag
     4021 ggcctgtggc tgctgaatga gctgaagaac tccgacgatc tgaacaaggt gaagctggcc
     4081 atcgacaatc agacctggct gaatttcgcc cagaacagga aaaggccggc ggccacgaaa
     4141 aaggccggcc aggcaaaaaa gaaaaaggga tcctacccat acgatgttcc agattacgct
     4201 tatccctacg acgtgcctga ttatgcatac ccatatgatg tccccgacta tgcctaa
//
