LOCUS       sequence-124381-         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of nucleotide free RhoA in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Garcia-Mata R, Wennerberg K, Arthur WT, Noren NK, Ellerbroek SM, 
            Burridge K
  TITLE     Analysis of activated GAPs and GEFs in cell lysates.
  JOURNAL   Methods Enzymol. 2006;406:425-37.
  PUBMED    16472675
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2006"; volume: "406"; pages: "425-37"
COMMENT     Sequence Label: pGEX-4T1-RhoA G17A
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..591
                     /codon_start=1
                     /product="human Rho family small GTPase RhoA"
                     /label=RhoA
                     /translation="MAAIRKKLVIVGDGACAKTCLLIVFSKDQFPEVYVPTVFENYVAD
                     IEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVK
                     HFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT
                     KDGVREVFEMATRAALQARRGKKKSGCLVL"
     primer_bind     complement(657..679)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ccggaattca tggctgccat ccggaagaaa ctggtgattg ttggtgatgg agcctgtgca
       61 aagacatgct tgctcatagt cttcagcaag gaccagttcc cagaggtgta tgtgcccaca
      121 gtgtttgaga actatgtggc agatatcgag gtggatggaa agcaggtaga gttggctttg
      181 tgggacacag ctgggcagga agattatgat cgcctgaggc ccctctccta cccagatacc
      241 gatgttatac tgatgtgttt ttccatcgac agccctgata gtttagaaaa catcccagaa
      301 aagtggaccc cagaagtcaa gcatttctgt cccaacgtgc ccatcatcct ggttgggaat
      361 aagaaggatc ttcggaatga tgagcacaca aggcgggagc tagccaagat gaagcaggag
      421 ccggtgaaac ctgaagaagg cagagatatg gcaaacagga ttggcgcttt tgggtacatg
      481 gagtgttcag caaagaccaa agatggagtg agagaggttt ttgaaatggc tacgagagct
      541 gctctgcaag ctagacgtgg gaagaaaaaa tctgggtgcc ttgtcttgtg actcgagcgg
      601 ccgcatcgtg actgactgac gatctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
      661 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
      721 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc gg
//
