LOCUS       sequence-124386-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-based vector for Marchantia polymorpha to express a floxed 
            gene, with an expression cassette for Cre-glucocorticoid receptor 
            fusion protein under the control of heat-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Nishihama R, Ishida S, Urawa H, Kamei Y, Kohchi T
  TITLE     Conditional Gene Expression/Deletion Systems for Marchantia 
            polymorpha Using its Own Heat-shock Promoter and the 
            Cre/loxP-mediated Site-specific Recombination.
  JOURNAL   Plant Cell Physiol. 2015 Jul 6. pii: pcv102.
  PUBMED    26148498
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Physiol. 2015 Jul 6. pii: pcv102."
COMMENT     Sequence Label: pMpGWB337
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(101..613,614..616,617..619))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     294..313
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     553..574
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    625..658
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        join(684..689,690..707,708..714)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    722..738
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     727..749
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 cggactggta gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
       61 tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct
      121 tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt
      181 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
      241 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
      301 agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg
      361 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg ggcgaagcac atcaggccgt
      421 agccgaaggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga
      481 acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact
      541 tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct
      601 cctcgccctt gctcaccatg gtggataact tcgtataatg tatgctatac gaagttatag
      661 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      721 attgtgagcg gataacaatt tcacacagga
//
