LOCUS       sequence-124391-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  receiving vector of ORFs for the construction of standard ORF parts 
            in YeastFab work.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: HcKan_O
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(44..146)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(147..178)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     misc_feature    235..255
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      complement(262..289)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(305..376)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atcgcggcct cgagcaagac gtttcccgtt gaatatggct cattttagct tccttagctc
       61 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
      121 agttggaacc tcttacgtgc cgatcaagtc aaaagcctcc ggtcggaggc ttttgacttt
      181 ctgctatgga ggtcaggtat gatttaaatg gtcagtcgtc tcggctaaga gaccctgcag
      241 cggccgctac tagtatataa acgcagaaag gcccacccga aggtgagcca gtgtgactct
      301 agtagagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
      361 cctttcgttt tatttgatgc ctggctctag tagcgatcta cactagcact atcagcgtta
      421 ttaagcaccg gtggagtgac gaccttcagc acgttcgtac tgttcaacga tggtgtagtc
      481 ttcgttgtgg gaggtgatgt ccagtttgat gtcggttttg taagcacccg gcagctgaac
      541 cggtttttta gccatgtagg tggttttaac ttcagcgtcg tagtgaccac cgtctttcag
      601 tttcagacgc attttgattt cacctttcag agcaccgtct tccgggtaca tacgttcggt
      661 ggaagcttcc caacccatgg tttttttctg cataaccgga ccgtcggacg ggaagttggt
      721 accacgcagt ttaactttgt agatgaactc accgtcttgc agggaggagt cctgggtaac
      781 ggtaacaaca ccaccgtctt cga
//
