LOCUS       sequence-124409-         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the N-terminal histidine-tagged DXH36 gene product upon 
            transfection into Rosetta 2 E. coli and induction with IPTG..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Vaughn JP, Creacy SD, Routh ED, Joyner-Butt C, Jenkins GS, Pauli S, 
            Nagamine Y, Akman SA
  TITLE     The DEXH protein product of the DHX36 gene is the major source of 
            tetramolecular quadruplex G4-DNA resolving activity in HeLa cell 
            lysates.
  JOURNAL   J Biol Chem. 2005 Nov 18;280(46):38117-20. Epub 2005 Sep 7.
  PUBMED    16150737
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2005-11-18"; volume: "280(46)"; pages: "38117-20. 
            Epub 2005 Sep 7"
COMMENT     Sequence Label: TriEx-4 DHX36
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        10..119
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             140..157
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             167..211
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             227..244
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             263..277
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 ttaatccggg acctttaatt caacccaaca caatatatta tagttaaata agaattatta
       61 tcaaatcatt tgtatattaa ttaaaatact atactgtaaa ttacatttta tttacaatca
      121 aaggagatat accatggcac accatcacca ccatcactct tctggtaaag aaaccgctgc
      181 tgcgaaattt gaacgccagc acatggactc gccaccgcct tctggtctgg tcccccgggg
      241 cagcgcaggt tctggtacga ttgatgacga cgacaaaatg agttatgact accatcagaa
      301 ctggggccgt gatgggggtc cccgcagctc cggtgggggc tatggagggg ggccagcagg
      361 gggtcatgga ggtaaccgag gctccggagg aggcggcggc ggcggagggg gtggtcgagg
      421 cggcaggggc cggcatcccg ggcacctgaa aggccgcgaa atcggcatgt ggtacgcgaa
      481 aaaacagggg cagaagaaca aggaagcgga gaggcaagag agagctgtag tacacatgga
      541 tgaacgacga gaagaacaaa ttgtacagtt actgaattct gttcaagcga agaatgataa
      601 agagtcagaa gcacagatat cctggtttgc tcctgaggat catggatacg gtactgaagt
      661 ttctactaag aacacaccat gctcagagaa caaacttgac atccaggaaa agaagttgat
      721 aaatcaagaa aaaaaaatgt ttagaatcag gaacagatca tatattgacc gagattctga
      781 gtatctcttg caagaaaatg aaccagatgg aactttagac caaaaattat tggaagattt
      841 acaaaagaaa aaaaatgacc ttcggtatat tgaaatgcag catttcagag aaaagctgcc
      901 ttcgtatgga atg
//
