LOCUS       sequence-124614-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of an ""INT"" construct_bearing three PP7 
            Stem-loops, targeting the GLuc reporter, in mammalian cells. U6 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pU6_(GLuc)_INT[3xPP7_SL]
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(294..313)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(477..494)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(477..493)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(486..508)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(702..721)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     821..843
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tagtcgactc tagacttaat taagaaaaaa agcaccgact cggtgccact ttttcaagtt
       61 gataacggac tagccttatt taaacttgct atgctgcgaa tacgagccag cgacgccata
      121 tcgtctgctg gagaccacgg agcccatatg ctctcgtgga gaggagcgac gccatatcgt
      181 ctgctccctc gtattcgcag catagctctt aaacatagtg agtcgtatct agatcggtgt
      241 ttcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggtaa
      301 gcatatgata gtccatttta aaacataatt ttaaaactgc aaactaccca agaaattatt
      361 actttctacg tcacgtattt tgtactaata tctttgtgtt tacagtcaaa ttaattccaa
      421 ttatctctct aacagccttg tatcgtatat gcaaatatga aggaatcatg ggaaacactg
      481 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
      541 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
      601 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
      661 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
      721 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      781 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
      841 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgccta
//
