LOCUS       sequence-124619-         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  complementation of yeast auxotrophic marker LEU2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Li D, Song JZ, Shan MH, Li SP, Liu W, Li H, Zhu J, Wang Y, Lin J, 
            Xie Z
  TITLE     A fluorescent tool set for yeast Atg proteins.
  JOURNAL   Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971.
  PUBMED    25998947
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2015"; volume: "11(6):954-60. doi"; pages: " 
            10.1080/15548627.2015.1040971"
COMMENT     Sequence Label: BS-Leu2
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..43
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     27..43
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tggagctcca ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat
       61 tcgatatcaa gcttattggt agcgggattt ggtgaagctg cgagattgat gaagaaagaa
      121 tttgacaacg accaagctca catcaaaaga ctatccgata aattagtcaa aggtctatta
      181 tccgctgaac ataccacgtt gaacggatct ccagatcatc gttatccagg gtgtgttaac
      241 gtttctttcg cctacgtgga aggagaatct ttattgatgg cactaaggga tatcgcatta
      301 tcctcgggtt cagcctgtac atctgcttcc ctagaacctt cttatgtttt acatgcgctg
      361 ggtaaggatg atgcattagc ccattcttcc atcagatttg gtattggtag atttagtact
      421 gaagaggagg tcgactacgt cgttaaggcc gtttctgaca gagtaaaatt cttgagggaa
      481 ctttcaccat tatgggaaat ggttcaagaa ggtattgact taaactccat caaatggtca
      541 ggtcattgag tgttttttat ttgttgtatt tttttttttt tagagaaaat cctccaatat
      601 ataaattagg aatcatagtt tcatgatttt ctgttacacc taactttttg tgtggtgccc
      661 tcctccttgt caatattaat gttaaagtgc aattcttttt ccttatcacg ttgagccatt
      721 agtatcaatt tgcttacctg tattccttta catcctcctt tttctccttc ttgataaatg
      781 tatgtagatt gcgtatatag tttcgtctac cctatgaaca tattcctcga gttaagcaag
      841 gattttctta acttcttcgg cgacagcatc accgacttcg gtggtactgt tggaaccacc
      901 taaatcacca gttctgatac ctgcatccaa aaccttttta actgcatctt caatggcctt
      961 accttcttca ggc
//
